Phospho-Tuberin (S939) Recombinant Rabbit Monoclonal Antibody [JE54-06]
Catalog# HA721762
Phospho-Tuberin (S939) Recombinant Rabbit Monoclonal Antibody [JE54-06]
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WB
-
Human
-
Mouse
-
unconjugated
Overview
Product Name
Phospho-Tuberin (S939) Recombinant Rabbit Monoclonal Antibody [JE54-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues around Ser939 of human tuberin.
Species Reactivity
Human (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB
Target Molecular Weight
Predicted band size: 201 kDa
Positive Control
SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate.
Conjugation
unconjugated
Clone Number
JE54-06
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors. Tuberin is directly phosphorylated at Thr1462 by Akt/PKB. Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity. In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation and that the human TSC complex is a direct biochemical target of the PI3K/Akt pathway. This data complements Drosophila genetics studies suggesting the possible involvement of the tuberin-hamartin complex in the PI3K/Akt mediated insulin pathway.
Background References
1. Huang Q et al. Loss of TSC1/TSC2 sensitizes immune checkpoint blockade in non-small cell lung cancer. Sci Adv. 2022 Feb
2. Alesi N et al. TSC2 regulates lysosome biogenesis via a non-canonical RAGC and TFEB-dependent mechanism. Nat Commun. 2021 Jul
Subcellular Location
Cytoplasm, Membrane.
Synonyms
FLJ43106 antibody
LAM antibody
OTTHUMP00000158940 antibody
OTTHUMP00000198394 antibody
OTTHUMP00000198395 antibody
PPP1R160 antibody
Protein phosphatase 1, regulatory subunit 160 antibody
TSC complex subunit 2 antibody
tsc2 antibody
TSC2_HUMAN antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-Tuberin (S939) on different lysates with Rabbit anti-Phospho-Tuberin (S939) antibody (HA721762) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate
Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour, then treated with λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 201 kDa
Observed band size: 250 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721762) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Tuberin Recombinant Rabbit Monoclonal Antibody [SC05-59]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
