Skip to content

TRIM56 Recombinant Rabbit Monoclonal Antibody [PSH02-17]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA721776

TRIM56 Recombinant Rabbit Monoclonal Antibody [PSH02-17]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TRIM56 Recombinant Rabbit Monoclonal Antibody [PSH02-17]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TRIM56 aa 350-755 (Q9BRZ2).

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 81 kDa

Positive Control

HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, 293T cell lysate, A375 cell lysate, COS-1 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse lung tissue lysate, HeLa, NIH/3T3, C2C12, PC-12.

Conjugation

unconjugated

Clone Number

PSH02-17

RRID

AB_3072888

Reactivity Data

WB FC
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Green Monkey
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity by mediating ubiquitination of CGAS and STING1. In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets STING1 to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta (By similarity). Also mediate monoubiquitination of CGAS, thereby promoting CGAS oligomerization and subsequent activation. Promotes also TNFalpha-induced NF-kappa-B signaling by mediating 'Lys-63'-linked ubiquitination TAK1, leading to enhanced interaction between TAK1 and CHUK/IKKalpha Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus. Acts as restriction factor of Zika virus through direct interaction with the viral RNA via its C-terminal region.

Background References

1. Yang X. 2022. TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein. J Exp Clin Cancer Res.41(1):336.

2. Chen Y. 2018. TRIM56 Suppresses Multiple Myeloma Progression by Activating TLR3/TRIF Signaling. Yonsei Med J. 59(1):43-50.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9BRZ2 Human

SwissProt: Q80VI1 Mouse

SwissProt: A0A8I6G8D1 Rat

Synonyms

A130009K11Rik antibody

DKFZp667O116 antibody

E3 ubiquitin-protein ligase TRIM56 antibody

FLJ35608 antibody

Gm452 antibody

MGC37358 antibody

OTTMUSP00000027392 antibody

RING finger protein 109 antibody

RNF109 antibody

TRI56_HUMAN antibody

Expand

Images

  • Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: MCF7 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: 293T cell lysate<br />Lane 5: A375 cell lysate<br />Lane 6: COS-1 cell lysate<br />Lane 7: C2C12 cell lysate<br />Lane 8: PC-12 cell lysate<br />Lane 9: Mouse lung tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 81 kDa<br />Observed band size: 81 kDa<br /><br />Exposure time: 3 minutes 54 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA721776) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: A375 cell lysate
    Lane 6: COS-1 cell lysate
    Lane 7: C2C12 cell lysate
    Lane 8: PC-12 cell lysate
    Lane 9: Mouse lung tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 81 kDa
    Observed band size: 81 kDa

    Exposure time: 3 minutes 54 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate (20 µg/Lane)<br />Lane 2: A549-si TRIM56 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 81 kDa<br />Observed band size: 81 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA721776) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate (20 µg/Lane)
    Lane 2: A549-si TRIM56 cell lysate (20 µg/Lane)

    Predicted band size: 81 kDa
    Observed band size: 81 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HeLa cells labeling TRIM56.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling TRIM56.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of NIH/3T3 cells labeling TRIM56.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of NIH/3T3 cells labeling TRIM56.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of C2C12 cells labeling TRIM56.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of C2C12 cells labeling TRIM56.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of PC-12 cells labeling TRIM56.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PC-12 cells labeling TRIM56.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"