Skip to content

TRIM56 Recombinant Rabbit Monoclonal Antibody [PSH02-17]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA721776

TRIM56 Recombinant Rabbit Monoclonal Antibody [PSH02-17]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TRIM56 Recombinant Rabbit Monoclonal Antibody [PSH02-17]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TRIM56 aa 350-755 (Q9BRZ2).

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 81 kDa

Positive Control

HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, 293T cell lysate, A375 cell lysate, COS-1 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse lung tissue lysate, HeLa, NIH/3T3, C2C12, PC-12.

Conjugation

unconjugated

Clone Number

PSH02-17

RRID

AB_3072888

Reactivity Data

WB FC
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Green Monkey
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity by mediating ubiquitination of CGAS and STING1. In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets STING1 to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta (By similarity). Also mediate monoubiquitination of CGAS, thereby promoting CGAS oligomerization and subsequent activation. Promotes also TNFalpha-induced NF-kappa-B signaling by mediating 'Lys-63'-linked ubiquitination TAK1, leading to enhanced interaction between TAK1 and CHUK/IKKalpha Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus. Acts as restriction factor of Zika virus through direct interaction with the viral RNA via its C-terminal region.

Background References

1. Yang X. 2022. TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein. J Exp Clin Cancer Res.41(1):336.

2. Chen Y. 2018. TRIM56 Suppresses Multiple Myeloma Progression by Activating TLR3/TRIF Signaling. Yonsei Med J. 59(1):43-50.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9BRZ2 Human

SwissProt: Q80VI1 Mouse

SwissProt: A0A8I6G8D1 Rat

Synonyms

A130009K11Rik antibody

DKFZp667O116 antibody

E3 ubiquitin-protein ligase TRIM56 antibody

FLJ35608 antibody

Gm452 antibody

MGC37358 antibody

OTTMUSP00000027392 antibody

RING finger protein 109 antibody

RNF109 antibody

TRI56_HUMAN antibody

Expand

Images

  • Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: MCF7 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: 293T cell lysate<br />Lane 5: A375 cell lysate<br />Lane 6: COS-1 cell lysate<br />Lane 7: C2C12 cell lysate<br />Lane 8: PC-12 cell lysate<br />Lane 9: Mouse lung tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 81 kDa<br />Observed band size: 81 kDa<br /><br />Exposure time: 3 minutes 54 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA721776) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: A375 cell lysate
    Lane 6: COS-1 cell lysate
    Lane 7: C2C12 cell lysate
    Lane 8: PC-12 cell lysate
    Lane 9: Mouse lung tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 81 kDa
    Observed band size: 81 kDa

    Exposure time: 3 minutes 54 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate (20 µg/Lane)<br />Lane 2: A549-si TRIM56 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 81 kDa<br />Observed band size: 81 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA721776) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate (20 µg/Lane)
    Lane 2: A549-si TRIM56 cell lysate (20 µg/Lane)

    Predicted band size: 81 kDa
    Observed band size: 81 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HeLa cells labeling TRIM56.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling TRIM56.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of NIH/3T3 cells labeling TRIM56.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of NIH/3T3 cells labeling TRIM56.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of C2C12 cells labeling TRIM56.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of C2C12 cells labeling TRIM56.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of PC-12 cells labeling TRIM56.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721776" style="font-weight: bold;text-decoration: underline;">HA721776</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PC-12 cells labeling TRIM56.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"