Human EGF Recombinant Rabbit Monoclonal Antibody [PSH02-19] - BSA and Azide free (Capture)
Overview
Product Name
Human EGF Recombinant Rabbit Monoclonal Antibody [PSH02-19] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human EGF aa 971-1023 (P01133).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Cap)
Molecular Weight
Predicted band size: 134 kDa
Positive Control
Recombinant Human EGF protein.
Conjugation
unconjugated
Clone Number
PSH02-19
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-20] to Human EGF (Detector) (HA721783). The reference range value is 1.23-300 pg/mL.
Target
Function
Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein originally found in rodents and humans. It has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. EGF binds to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation of the receptor on tyrosine residues. It has been detected in nearly all body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF, TGFα and vaccinia virus growth factor exhibit 30-40% amino acid homology. Several additional members of the EGF/TGF family have been described; these include Cripto, Amphiregulin and the heparin-binding EGF-like growth factor. Amphiregulin and the heparin-binding EGF-like growth factor both bind to the EGF receptor.
Background References
1. Zhang X et al. Hypoxia-inducible factor-1a mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions. Oncol Lett 12:1034-1040 (2016).
2. Guo Z et al. Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and iTRAQ quantification. J Transl Med 13:371 (2015).
Subcellular Location
Membrane.
UNIPROT
Synonyms
Beta urogastrone antibody
beta-urogastrone antibody
EGF antibody
EGF_HUMAN antibody
Epidermal growth factor antibody
HOMG4 antibody
OTTHUMP00000219721 antibody
OTTHUMP00000219722 antibody
Pro epidermal growth factor antibody
URG antibody
ExpandImages
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![Sandwich ELISA analysis of Human EGF matched pair antibodies.<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721782" style="font-weight: bold;text-decoration: underline;">HA721782</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human EGF protein starting from 300 pg/ml to 0 pg/ml and detect antibody [PSH02-20]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721782_1.jpg?v=20260522195651)
Sandwich ELISA analysis of Human EGF matched pair antibodies.
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721782) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human EGF protein starting from 300 pg/ml to 0 pg/ml and detect antibody [PSH02-20]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Biotin Conjugated Human EGF Recombinant Rabbit Monoclonal Antibody [PSH02-20] - Detector
Application: ELISA(Det)
Reactivity: Human,Cynomolgus monkey
Conjugate: Biotin
EGF Recombinant Rabbit Monoclonal Antibody [JA14-15]
Application: WB,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
