Rat IL-10 Recombinant Rabbit Monoclonal Antibody [PSH02-22] - BSA and Azide free (Detector)
Overview
Product Name
Rat IL-10 Recombinant Rabbit Monoclonal Antibody [PSH02-22] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Rat IL-10 aa 19-178 (P29456).
Species Reactivity
Rat
Validated Applications
ELISA(Det)
Molecular Weight
Predicted band size: 20 kDa
Positive Control
Recombinant Rat IL-10 protein (HA210714).
Conjugation
unconjugated
Clone Number
PSH02-22
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-21] to Rat IL-10 (Capture) (HA721786). The reference range value is 13.7-10,000 pg/mL.
Target
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.
Background References
1. Cai W et al. Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice. J Clin Invest. 2022 Aug
2. Yang L et al. Depression-like behavior associated with E/I imbalance of mPFC and amygdala without TRPC channels in mice of knockout IL-10 from microglia. Brain Behav Immun. 2021 Oct
Subcellular Location
Secreted
UNIPROT
Synonyms
CSIF antibody
Cytokine synthesis inhibitory factor antibody
GVHDS antibody
IL 10 antibody
IL-10 antibody
IL10 antibody
IL10_HUMAN antibody
IL10A antibody
Interleukin 10 antibody
Interleukin-10 antibody
ExpandImages
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![Sandwich ELISA analysis of Rat IL-10 matched pair antibodies.<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721786" style="font-weight: bold;text-decoration: underline;">HA721786</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IL-10 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody [PSH02-22]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721787_1.png?v=20260509231357)
Sandwich ELISA analysis of Rat IL-10 matched pair antibodies.
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721786) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IL-10 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody [PSH02-22]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"