TXNRD2 Recombinant Rabbit Monoclonal Antibody [PSH02-27]
Overview
Product Name
TXNRD2 Recombinant Rabbit Monoclonal Antibody [PSH02-27]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TXNRD2 aa 1-524 / 524.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 57 kDa
Positive Control
HEK-293 cell lysate, HeLa cell lysate, A431 cell lysate, K-562 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Human kidney tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human prostate tissue.
Conjugation
unconjugated
Clone Number
PSH02-27
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
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| Mouse |
|
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| Rat |
|
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:200
Target
Function
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene.
Background References
1. Aboobakar IF et al. Mitochondrial TXNRD2 and ME3 Genetic Risk Scores Are Associated with Specific Primary Open-Angle Glaucoma Phenotypes. Ophthalmology. 2023 Jul
2. Kondkar AA et al. Lack of Association Between Polymorphisms in TXNRD2 and LMX1B and Primary Open-Angle Glaucoma in a Saudi Cohort. Front Genet. 2021 Aug
Subcellular Location
Mitochondrion.
Synonyms
mitochondrial antibody
Selenoprotein Z antibody
SelZ antibody
Thioredoxin reductase 2 antibody
Thioredoxin reductase 2 mitochondrial antibody
Thioredoxin reductase 3 antibody
Thioredoxin reductase beta antibody
Thioredoxin reductase TR3 antibody
TR 3 antibody
TR antibody
ExpandImages
-
Western blot analysis of TXNRD2 on different lysates with Rabbit anti-TXNRD2 antibody (HA721807) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat heart tissue lysate (40 µg/Lane)
Lane 7: Mouse liver tissue lysate (40 µg/Lane)
Lane 8: Rat liver tissue lysate (40 µg/Lane)
Lane 9: Human kidney tissue lysate (40 µg/Lane)
Lane 10: Mouse kidney tissue lysate (40 µg/Lane)
Lane 11: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721807) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-TXNRD2 antibody (HA721807) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721807) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Citation
-
Selenium nanoparticles alleviate ulcerative colitis by regulating macrophage mitochondrial dynamics
Journal: Free Radical Biology And Medicine
DOI: 10.1016/j.freeradbiomed.2026.06.040
IF: 8
Application: WB
Reactivity: Human
Publish date: 2026 Jun
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"