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MTH1 Recombinant Rabbit Monoclonal Antibody [PSH02-40]

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Specification

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Catalog# HA721817

MTH1 Recombinant Rabbit Monoclonal Antibody [PSH02-40]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

MTH1 Recombinant Rabbit Monoclonal Antibody [PSH02-40]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MTH1 aa 1-156 / 156 (P36639).

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 18 kDa

Positive Control

HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, K-562 cell lysate, A549 cell lysate, MCF7 cell lysate, SW480 cell lysate, THP-1 cell lysate, SK-OV-3 cell lysate, U-2 OS cell lysate, human breast cancer tissue, NIH/3T3 cell lysate, C2C12 cell lysate, bEnd.3 cell lysate, RAW264.7 cell lysate, F9 cell lysate, Neuro-2a cell lysate, C6 cell lysate, L6 cell lysate, PC-12 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, mouse testis tissue lysate, rat testis tissue lysate, human stomach cancer tissue, human thymus tissue, rat thymus tissue, Jurkat.

Conjugation

unconjugated

Clone Number

PSH02-40

RRID

AB_3072929

Reactivity Data

WB IHC-P FC
Human
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Not Recommended
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200~1:1,000

  • FC

  • 1:1,000

Target

Function

Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool. Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP. Has also a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP. Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A . Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA . Through this antimutagenic activity protects cells from oxidative stress .

Background References

1. Duan J.et al. The role of miR-485-5p/NUDT1 axis in gastric cancer. Cancer Cell Int. 2017 Oct 17;17:92.

2. Ding Y.et al. MTH1 protects platelet mitochondria from oxidative damage and regulates platelet function and thrombosis. Nat Commun. 2023 Aug 10;14(1):4829.

Subcellular Location

Cytoplasm, cytosol,Mitochondrion matrix,Nucleus,Mitochondrion matrix

UNIPROT

SwissProt: P36639 Human

SwissProt: P53368 Mouse

SwissProt: P53369 Rat

Synonyms

2-hydroxy-dATP diphosphatase antibody

7 8 dihydro 8 oxoguanine triphosphatase antibody

7 antibody

8 oxo 7 8 dihydrodeoxyguanosine triphosphatase antibody

8 oxo 7 8 dihydroguanosine triphosphatase antibody

8 oxo dGTPase antibody

8-dihydro-8-oxoguanine triphosphatase antibody

8-oxo-dGTPase antibody

8ODP_HUMAN antibody

MTH 1 antibody

Expand

Images

  • Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: HepG2 cell lysate<br />Lane 5: K-562 cell lysate<br />Lane 6: A549 cell lysate<br />Lane 7: MCF7 cell lysate<br />Lane 8: SW480 cell lysate<br />Lane 9: THP-1 cell lysate<br />Lane 10: SK-OV-3 cell lysate<br />Lane 11: U-2 OS cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 18 kDa<br />Observed band size: 18 kDa<br /><br />Exposure time: 5 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: K-562 cell lysate
    Lane 6: A549 cell lysate
    Lane 7: MCF7 cell lysate
    Lane 8: SW480 cell lysate
    Lane 9: THP-1 cell lysate
    Lane 10: SK-OV-3 cell lysate
    Lane 11: U-2 OS cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

    Exposure time: 5 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: NIH/3T3 cell lysate <br />Lane 3: C2C12 cell lysate <br />Lane 4: bEnd.3 cell lysate<br />Lane 5: RAW264.7 cell lysate<br />Lane 6: F9 cell lysate<br />Lane 7: Neuro-2a cell lysate<br />Lane 8: C6 cell lysate<br />Lane 9: L6 cell lysate<br />Lane 10: PC-12 cell lysate<br />Lane 11: Mouse thymus tissue lysate<br />Lane 12: Rat thymus tissue lysate<br />Lane 13: Mouse testis tissue lysate<br />Lane 14: Rat testis tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 18 kDa<br />Observed band size: 18 kDa<br /><br />Exposure time: 5 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: C2C12 cell lysate
    Lane 4: bEnd.3 cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: F9 cell lysate
    Lane 7: Neuro-2a cell lysate
    Lane 8: C6 cell lysate
    Lane 9: L6 cell lysate
    Lane 10: PC-12 cell lysate
    Lane 11: Mouse thymus tissue lysate
    Lane 12: Rat thymus tissue lysate
    Lane 13: Mouse testis tissue lysate
    Lane 14: Rat testis tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

    Exposure time: 5 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-MTH1 antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-MTH1 antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-MTH1 antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293-si NT cell lysate<br />Lane 2: HEK-293-si MTH1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 18 kDa<br />Observed band size: 18 kDa<br /><br />Exposure time: 1 minute 34 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution.

    Lane 1: HEK-293-si NT cell lysate
    Lane 2: HEK-293-si MTH1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

    Exposure time: 1 minute 34 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of Jurkat cells labeling MTH1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721817" style="font-weight: bold;text-decoration: underline;">HA721817</a>,1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Jurkat cells labeling MTH1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721817,1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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