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MAG Recombinant Rabbit Monoclonal Antibody [PSH02-41]

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Specification

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Catalog# HA721818

MAG Recombinant Rabbit Monoclonal Antibody [PSH02-41]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IP

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

MAG Recombinant Rabbit Monoclonal Antibody [PSH02-41]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MAG aa 1-536 / 626.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IF-Tissue, IP, IHC-Fr

Molecular Weight

Predicted band size: 69 kDa

Positive Control

Mouse brain(no heat) tissue lysate, mouse cerebellum tissue lysate, rat brain(no heat) tissue lysate, rat cerebellum tissue lysate, human brain tissue, human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue, mouse hippocampus tissue, rat brain tissue, rat cerebellum tissue, rat hippocampus tissue.

Conjugation

unconjugated

Clone Number

PSH02-41

RRID

AB_3072930

Reactivity Data

WB IHC-P IF-Tissue IP IHC-Fr
Human
Tested Tested
Mouse
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Cynomolgus Monkey
Published
Published
Published
Published
Published
Pig
Published
Published
Published
Published
Published

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:2,000-1:5,000

  • IF-Tissue

  • 1:200-1:1,000

  • IP

  • 1-2μg/sample

  • IHC-Fr

  • 1:500-1:1,000

Target

Function

Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).

Background References

1. Zis P, Rao DG, Hoggard N, et al. Anti-MAG associated cerebellar ataxia and response to rituximab. J Neurol. 2018 Jan;265(1):115-118.

2. Quarles RH. Myelin-associated glycoprotein (MAG): past, present and beyond. J Neurochem. 2007 Mar;100(6):1431-48.

Subcellular Location

Cell membrane, Membrane raft.

UNIPROT

SwissProt: P20916 Human

SwissProt: P20917 Mouse

SwissProt: P07722 Rat

Synonyms

GMA antibody

MAG antibody

MAG_HUMAN antibody

Myelin associated glycoprotein antibody

Myelin-associated glycoprotein antibody

S MAG antibody

S-MAG antibody

Sialic acid binding Ig like lectin 4A antibody

Sialic acid binding immunoglobulin like lectin 4A antibody

Siglec 4a antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500 (MAG, <a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>, red); 1:500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1: 500 (MAG, HA721818, red); 1:500 (Iba1, HA601376, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-MAG antibody (HA721818) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-MAG antibody (HA721818) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:200

    Application: IF-tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1:200

  • Application: IF-tissue<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:200

    Application: IF-tissue

    Species: Rat

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1:200

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of MAG on different lysates with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: Mouse cerebellum tissue lysate (20 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (negative) (20 µg/Lane)<br />Lane 3: Rat cerebellum tissue lysate (20 µg/Lane)<br />Lane 4: Rat liver tissue lysate (negative) (20 µg/Lane)<br /><br />Predicted band size: 69 kDa<br />Observed band size: 100 kDa<br />Exposure time: 2 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of MAG on different lysates with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: Mouse cerebellum tissue lysate (20 µg/Lane)
    Lane 2: Mouse liver tissue lysate (negative) (20 µg/Lane)
    Lane 3: Rat cerebellum tissue lysate (20 µg/Lane)
    Lane 4: Rat liver tissue lysate (negative) (20 µg/Lane)

    Predicted band size: 69 kDa
    Observed band size: 100 kDa
    Exposure time: 2 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721818) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of MAG on different lysates with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane)<br />Lane 2: Mouse cerebellum tissue lysate (20 µg/Lane)<br />Lane 3: Mouse liver tissue lysate (negative) (20 µg/Lane)<br />Lane 4: Rat brain tissue lysate (no heat) (20 µg/Lane)<br />Lane 5: Rat cerebellum tissue lysate (20 µg/Lane)<br />Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 69 kDa<br />Observed band size: 100 kDa<br />Exposure time: 42 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of MAG on different lysates with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane)
    Lane 2: Mouse cerebellum tissue lysate (20 µg/Lane)
    Lane 3: Mouse liver tissue lysate (negative) (20 µg/Lane)
    Lane 4: Rat brain tissue lysate (no heat) (20 µg/Lane)
    Lane 5: Rat cerebellum tissue lysate (20 µg/Lane)
    Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 69 kDa
    Observed band size: 100 kDa
    Exposure time: 42 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721818) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver (negative) tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver (negative) tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse liver (negative) tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse liver (negative) tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat liver (negative) tissue with Rabbit anti-MAG antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat liver (negative) tissue with Rabbit anti-MAG antibody (HA721818) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721818) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MAG was immunoprecipitated from 0.2 mg mouse brain tissue lysate with <a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (<a href="/products/NBI01H" style="font-weight: bold;text-decoration: underline;">NBI01H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Mouse brain tissue lysate (input)<br />Lane 2: <a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a> IP in mouse brain tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a> in mouse brain tissue lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 32 seconds; ECL: K1801

    MAG was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA721818 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721818 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Mouse brain tissue lysate (input)
    Lane 2: HA721818 IP in mouse brain tissue lysate
    Lane 3: Rabbit IgG instead of HA721818 in mouse brain tissue lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 32 seconds; ECL: K1801

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