Galectin 9 Recombinant Rabbit Monoclonal Antibody [PSH02-44]
Catalog# HA721821
Galectin 9 Recombinant Rabbit Monoclonal Antibody [PSH02-44]
-
WB
-
FC
-
Human
-
Mouse
-
HA750809
不含抗保成分
-
unconjugated
Overview
Product Name
Galectin 9 Recombinant Rabbit Monoclonal Antibody [PSH02-44]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Galectin 9 aa 51-100 / 355.
Species Reactivity
Human, Mouse
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 40 kDa
Positive Control
THP-1 cell lysate, PANC-1 cell lysate, HL-60 cell lysate, A549 cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, THP-1.
Conjugation
unconjugated
Clone Number
PSH02-44
RRID
Reactivity Data
| WB | FC | |
|---|---|---|
| Human |
|
|
| Mouse |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
FC
-
1:1,000
Target
Function
The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide. Multiple alternatively spliced transcript variants have been found for this gene. Galectin-9 is one of the most studied ligands for HAVCR2 (TIM-3) and is expressed on various tumor cells. However, it can also interact with other proteins (CLEC7A, CD137, CD40). For example, an interaction with CD40 on T-cells inhibits their proliferation and induces cell death. Galectin-9 also has important cytoplasmic, intracellular functions and controls AMPK in response to lysosomal damage that can occur upon exposure to endogenous and exogenous membrane damaging agents such as crystalline silica, cholesterol crystals, microbial toxins, proteopathic aggregates such as tau fibrils and amyloids, and signaling pathways inducing lysosomal permeabilization such as those initiated by TRAIL. Mild lysosomal damage, such as that caused by the anti-diabetes drug metformin may contribute to the therapeutic action of metformin by activating AMPK. The mechanism of how Galectin-9 activates AMPK involves recognition of exposed lysosomal lumenal glycoproteins such as LAMP1, LAMP2, SCRAB2, TMEM192, etc., repulsion of deubiquitinating enzyme USP9X, increased K63 ubiquitination of TAK1 (MAP3K7) kinase, which in turn phopshorylates AMPK and activates it. This signaling cascade directly links Galectin-9 intracellular function with ubiqutin systems. Galectin-9, through its regulation of AMPK, a kinase that negatively regulates mTOR, cooperates with Galectin-8-based effects to inactivate mTOR downstream of the lysosomal damaging agents and conditions.
Background References
1. Morishita A et al. Galectin-9 in Gastroenterological Cancer. Int J Mol Sci. 2023 Mar
2. Yang R et al. Galectin-9 interacts with PD-1 and TIM-3 to regulate T cell death and is a target for cancer immunotherapy. Nat Commun. 2021 Feb
Subcellular Location
Cytoplasm, Nucleus, Secreted.
Synonyms
36 kDa beta-galactoside-binding lectin antibody
Ecalectin antibody
Gal-9 antibody
galectin 9 antibody
Galectin-9 antibody
galectin9 antibody
HOM HD 21 antibody
HOMHD21 antibody
HUAT antibody
Lectin galactoside binding soluble 9 antibody
ExpandImages
-
☑ Relative expression (RE)
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA721821) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: PC-3 cell lysate (low expression)
Lane 3: PANC-1 cell lysate (low expression)
Lane 4: HL-60 cell lysate
Lane 5: A549 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: RAW264.7 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 36 kDa
Exposure time: 2 minutes 7 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721821) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of THP-1 cells labeling Galectin 9.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721821, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"