CD276 Recombinant Rabbit Monoclonal Antibody [PSH02-46] (HA721823)
Catalog# HA721823
Rabbit Monoclonal to CD276
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WB
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IF-Cell
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FC
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Human
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: LoVo cell lysate
Lane 5: U-2 OS cell lysate
Lane 6: LNCaP cell lysate
Lane 7: SH-SY5Y cell lysate
Lane 8: THP-1 cell lysate
Lane 9: HCT 116 cell lysate
Lane 10: Raji cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 100 kDa
Exposure time: 42 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721823) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD276 Recombinant Rabbit Monoclonal Antibody [PSH02-46] (HA721823)
Immunogen
Full length recombinant protein within human cd276.
Host
Rabbit
Positive Control
HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, LoVo cell lysate, U-2 OS cell lysate, LNCaP cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, HCT 116 cell lysate, THP-1, MCF7.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH02-46
RRID
APPLICATION DILUTION
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WB
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1:2,000-5,000
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IF-Cell
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1:100
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 57 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD276
SYNONYMS
4Ig B7 H3 antibody;4Ig-B7-H3 antibody;AU016588 antibody;B7 H3 antibody;B7 homolog 3 antibody;B7-H3 antibody;B7H3 antibody;B7RP-2 antibody;CD_antigen=CD276 antibody;CD276 antibody;CD276 antigen antibody;CD276 molecule antibody;CD276_HUMAN antibody;Costimulatory molecule antibody;Flags: Precursor antibody;PSEC0249 antibody;UNQ309/PRO352 antibody
SUBCELLULAR LOCATION
Membrane.
FUNCTION
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
CITATIONS
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Wu Yulu,et al
B7-H3 suppresses CD8+ T cell immunologic function through reprogramming glycolytic metabolism
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WB
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Human
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Citation
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