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GSTA4 Recombinant Rabbit Monoclonal Antibody [JE61-65]

Usd: 385

Specification

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Catalog# HA721825

GSTA4 Recombinant Rabbit Monoclonal Antibody [JE61-65]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

GSTA4 Recombinant Rabbit Monoclonal Antibody [JE61-65]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human GSTA4 aa 1-100.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 26 kDa

Positive Control

HEK-293 cell lysate, JAR cell lysate, human liver tissue lysate, human brain tissue lysate, human kidney tissue lysate.

Conjugation

unconjugated

Clone Number

JE61-65

RRID

AB_3072937

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis.

Background References

1. Ucche S et al. GSTA4 Governs Melanoma Immune Resistance and Metastasis. Mol Cancer Res. 2023 Jan

2. Park HJ et al. Effects of Gsta4 deficiency on age-related cochlear pathology and hearing loss in mice. Exp Gerontol. 2020 May

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O15217 Human

Synonyms

DKFZp686D21185 antibody

Glutathione S alkyltransferase A4 antibody

Glutathione S aralkyltransferase A4 antibody

Glutathione S aryltransferase A4 antibody

Glutathione S transferase A4 4 antibody

Glutathione S transferase A4 antibody

Glutathione S transferase alpha 4 antibody

Glutathione S-transferase A4 antibody

Glutathione S-transferase A4-4 antibody

Glutathione transferase A4 4 antibody

Expand

Images

  • Western blot analysis of GSTA4 on different lysates with Rabbit anti-GSTA4 antibody (<a href="/products/HA721825" style="font-weight: bold;text-decoration: underline;">HA721825</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (40 µg/Lane)<br />Lane 2: JAR cell lysate (40 µg/Lane)<br />Lane 3: Human liver tissue lysate (40 µg/Lane)<br />Lane 4: Human brain tissue lysate (30 µg/Lane)<br />Lane 5: Human kidney tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 26 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 1 minute 59 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721825" style="font-weight: bold;text-decoration: underline;">HA721825</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GSTA4 on different lysates with Rabbit anti-GSTA4 antibody (HA721825) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate (40 µg/Lane)
    Lane 2: JAR cell lysate (40 µg/Lane)
    Lane 3: Human liver tissue lysate (40 µg/Lane)
    Lane 4: Human brain tissue lysate (30 µg/Lane)
    Lane 5: Human kidney tissue lysate (30 µg/Lane)

    Predicted band size: 26 kDa
    Observed band size: 25 kDa

    Exposure time: 1 minute 59 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721825) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of GSTA4 on different lysates with Rabbit anti-GSTA4 antibody (<a href="/products/HA721825" style="font-weight: bold;text-decoration: underline;">HA721825</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-GSTA4 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721825" style="font-weight: bold;text-decoration: underline;">HA721825</a>) at 1/2,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of GSTA4 on different lysates with Rabbit anti-GSTA4 antibody (HA721825) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-GSTA4 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721825) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"