CD33 Recombinant Rabbit Monoclonal Antibody [PSH02-51] (HA721827)
Catalog# HA721827
Rabbit Monoclonal to CD33
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD33 on different lysates with Rabbit anti-CD33 antibody (HA721827) at 1/2,000 dilution.
Lane 1: TF-1 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: SK-MEL-28 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: TF-1 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: SK-MEL-28 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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