Human VE Cadherin Recombinant Rabbit Monoclonal Antibody [PSH02-62] - BSA and Azide free (Detector)
Overview
Product Name
Human VE Cadherin Recombinant Rabbit Monoclonal Antibody [PSH02-62] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human VE-cadherin aa 48-599 (P33151).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Molecular Weight
Predicted band size: 87.5 kDa
Positive Control
Recombinant Human VE Cadherin protein (HA210635).
Conjugation
unconjugated
Clone Number
PSH02-62
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-61] to Human VE Cadherin (Capture) (HA721838) and recombinant standard Human VE Cadherin (HA210635) as the standard. The reference range value is 0.63-153.09 ng/mL.
Target
Function
VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.
Background References
1. Shimoyama Y., Tsujimoto G., Kitajima M., Natori M. Identification of three human type-II classic cadherins and frequent heterophilic interactions between different subclasses of type-II classic cadherins. Biochem. J. 349:159-167 (2000)
2. Lampugnani M.G., Orsenigo F., Rudini N., Maddaluno L., Boulday G., Chapon F., Dejana E. CCM1 regulates vascular-lumen organization by inducing endothelial polarity. J. Cell Sci. 123:1073-1080 (2010)
3. Brasch J., Harrison O.J., Ahlsen G., Carnally S.M., Henderson R.M., Honig B., Shapiro L. Structure and binding mechanism of vascular endothelial cadherin: a divergent classical cadherin. J. Mol. Biol. 408:57-73 (2011)
Subcellular Location
Cell junction. Cell membrane. Membrane.
UNIPROT
Synonyms
7B 4 antibody
7B4 antibody
7B4 antigen antibody
CADH5_HUMAN antibody
Cadherin 5 antibody
Cadherin 5 type 2 antibody
Cadherin 5, type 2 (vascular endothelium) antibody
Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
cadherin, vascular endothelial antibody
cadherin, vascular endothelial, 1 antibody
ExpandImages
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![Sandwich ELISA analysis of human Cadherin-5 matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA721838" style="font-weight: bold;text-decoration: underline;">HA721838</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Cadherin-5 protein (<a href="/products/HA210635" style="font-weight: bold;text-decoration: underline;">HA210635</a>) starting from 20000 pg/ml to 0 pg/ml and detect antibody [PSH02-62] -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721839_1.jpg?v=20260429161658)
Sandwich ELISA analysis of human Cadherin-5 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA721838) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Cadherin-5 protein (HA210635) starting from 20000 pg/ml to 0 pg/ml and detect antibody [PSH02-62] -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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