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gamma Adaptin Recombinant Rabbit Monoclonal Antibody [PSH02-65]

Usd: 385

Specification

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Catalog# HA721843

gamma Adaptin Recombinant Rabbit Monoclonal Antibody [PSH02-65]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

gamma Adaptin Recombinant Rabbit Monoclonal Antibody [PSH02-65]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human gamma Adaptin aa 623-822 / 822.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 91 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, HepG2 cell lysate, Raji cell lysate, MCF7 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, mouse liver tissue lysate, rat kidney tissue lysate, rat liver tissue lysate, mouse kidney tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH02-65

RRID

AB_3072955

Reactivity Data

WB IHC-P
Human
Tested Tested
Not Recommended
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. In association with AFTPH/aftiphilin in the aftiphilin/p200/gamma-synergin complex, involved in the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes.

Background References

1. Usmani MA, Ahmed ZM, Magini P, et al. De novo and bi-allelic variants in AP1G1 cause neurodevelopmental disorder with developmental delay, intellectual disability, and epilepsy. Am J Hum Genet. 2021 Jul 1;108(7):1330-1341.

2. Tao X, Lu Y, Qiu S, et al. AP1G1 is involved in cetuximab-mediated downregulation of ASCT2-EGFR complex and sensitization of human head and neck squamous cell carcinoma cells to ROS-induced apoptosis. Cancer Lett. 2017 Nov 1; 408: 33-42.

Subcellular Location

Golgi apparatus, Cytoplasmic vesicle, clathrin-coated vesicle membrane, Cytoplasm, perinuclear region,

UNIPROT

SwissProt:O43747 Human

SwissProt:P22892 Mouse

SwissProt:A0A8I5Y697 Rat

Synonyms

Adapter-related protein complex 1 subunit gamma-1 antibody

Adaptor protein complex AP 1 gamma 1 subunit antibody

Adaptor protein complex AP 1 subunit gamma 1 antibody

Adaptor protein complex AP-1 subunit gamma-1 antibody

Adaptor related protein complex 1 gamma 1 subunit antibody

ADTG antibody

AP 1 complex subunit gamma 1 antibody

AP-1 complex subunit gamma-1 antibody

AP1G1 antibody

AP1G1_HUMAN antibody

Expand

Images

  • Western blot analysis of gamma Adaptin on different lysates with Rabbit anti-gamma Adaptin antibody (<a href="/products/HA721843" style="font-weight: bold;text-decoration: underline;">HA721843</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: 293T cell lysate (20 µg/Lane)<br />Lane 3: Jurkat cell lysate (20 µg/Lane)<br />Lane 4: HepG2 cell lysate (20 µg/Lane)<br />Lane 5: Raji cell lysate (20 µg/Lane)<br />Lane 6: MCF7 cell lysate (20 µg/Lane)<br />Lane 7: A549 cell lysate (20 µg/Lane)<br />Lane 8: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 9: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 10: PC-12 cell lysate (20 µg/Lane)<br />Lane 11: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 12: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 13: Rat kidney tissue lysate (40 µg/Lane)<br />Lane 14: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 91 kDa<br />Observed band size: 91 kDa<br /><br />Exposure time: 1 minute 46 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721843" style="font-weight: bold;text-decoration: underline;">HA721843</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of gamma Adaptin on different lysates with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: 293T cell lysate (20 µg/Lane)
    Lane 3: Jurkat cell lysate (20 µg/Lane)
    Lane 4: HepG2 cell lysate (20 µg/Lane)
    Lane 5: Raji cell lysate (20 µg/Lane)
    Lane 6: MCF7 cell lysate (20 µg/Lane)
    Lane 7: A549 cell lysate (20 µg/Lane)
    Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 9: RAW264.7 cell lysate (20 µg/Lane)
    Lane 10: PC-12 cell lysate (20 µg/Lane)
    Lane 11: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 12: Mouse liver tissue lysate (40 µg/Lane)
    Lane 13: Rat kidney tissue lysate (40 µg/Lane)
    Lane 14: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 91 kDa
    Observed band size: 91 kDa

    Exposure time: 1 minute 46 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721843) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-gamma Adaptin antibody (<a href="/products/HA721843" style="font-weight: bold;text-decoration: underline;">HA721843</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721843" style="font-weight: bold;text-decoration: underline;">HA721843</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-gamma Adaptin antibody (<a href="/products/HA721843" style="font-weight: bold;text-decoration: underline;">HA721843</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721843" style="font-weight: bold;text-decoration: underline;">HA721843</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of gamma Adaptin on different lysates with Rabbit anti-gamma Adaptin antibody (<a href="/products/HA721843" style="font-weight: bold;text-decoration: underline;">HA721843</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293-si NT cell lysate<br />Lane 2: HEK-293-si gamma Adaptin cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 91 kDa<br />Observed band size: 91 kDa<br /><br />Exposure time: 2 minutes 18 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721843" style="font-weight: bold;text-decoration: underline;">HA721843</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of gamma Adaptin on different lysates with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/1,000 dilution.

    Lane 1: HEK-293-si NT cell lysate
    Lane 2: HEK-293-si gamma Adaptin cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 91 kDa
    Observed band size: 91 kDa

    Exposure time: 2 minutes 18 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721843) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"