Skip to content

c17orf74 Recombinant Rabbit Monoclonal Antibody [PSH02-66]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA721844

c17orf74 Recombinant Rabbit Monoclonal Antibody [PSH02-66]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

c17orf74 Recombinant Rabbit Monoclonal Antibody [PSH02-66]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human c17orf74 aa 202-501 / 501.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 57 kDa

Positive Control

Human testis tissue, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

PSH02-66

RRID

AB_3072956

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Not Recommended
Tested Tested
Rat
Not Recommended
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

SPEM family member 2.

Background References

暂无

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q0P670 Human

SwissProt: Q8C5U4 Mouse

SwissProt: Q68FV4 Rat

Synonyms

Uncharacterized protein SPEM2

SPEM2

C17orf74

SPEM family member 2

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of c17orf74 on different lysates with Rabbit anti-c17orf74 antibody (<a href="/products/HA721844" style="font-weight: bold;text-decoration: underline;">HA721844</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293-NT cell lysate<br />Lane 2: HEK-293-OE-c17orf74 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 57 kDa<br /><br />Exposure time: 24 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721844" style="font-weight: bold;text-decoration: underline;">HA721844</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of c17orf74 on different lysates with Rabbit anti-c17orf74 antibody (HA721844) at 1/1,000 dilution.

    Lane 1: HEK-293-NT cell lysate
    Lane 2: HEK-293-OE-c17orf74 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

    Exposure time: 24 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721844) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-c17orf74 antibody (<a href="/products/HA721844" style="font-weight: bold;text-decoration: underline;">HA721844</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721844" style="font-weight: bold;text-decoration: underline;">HA721844</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-c17orf74 antibody (HA721844) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721844) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-c17orf74 antibody (<a href="/products/HA721844" style="font-weight: bold;text-decoration: underline;">HA721844</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721844" style="font-weight: bold;text-decoration: underline;">HA721844</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-c17orf74 antibody (HA721844) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721844) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-c17orf74 antibody (<a href="/products/HA721844" style="font-weight: bold;text-decoration: underline;">HA721844</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721844" style="font-weight: bold;text-decoration: underline;">HA721844</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-c17orf74 antibody (HA721844) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721844) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"