Mouse VEGF Recombinant Rabbit Monoclonal Antibody [PSH02-73] - BSA and Azide free (Detector)
Overview
Product Name
Mouse VEGF Recombinant Rabbit Monoclonal Antibody [PSH02-73] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant full length protein.
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
Recombinant Mouse VEGF protein
Conjugation
unconjugated
Clone Number
PSH02-73
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-72] to Mouse VEGF (Capture) (HA721850). The reference range value is 13.72-10,000 pg/mL.
Target
Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression continues in kidney glomeruli and choroid plexus in adults.
Background References
1. He W., Bai G., Zhou H., Wei N., White N.M., Lauer J., Liu H., Shi Y., Dumitru C.D., Lettieri K., Shubayev V., Jordanova A., Guergueltcheva V., Griffin P.R., Burgess R.W., Pfaff S.L., Yang X.L. CMT2D neuropathy is linked to the neomorphic binding activity of glycyl- tRNA synthetase. Nature 526:710-714 (2015)
2. Katsman M., Azriel A., Horev G., Reizel Y., Levi B.Z. N-VEGF, the Autoregulatory Arm of VEGF-A. Cells 11:0-0 (2022)
Subcellular Location
Secreted.
UNIPROT
Synonyms
Folliculostellate cell-derived growth factor antibody
Glioma-derived endothelial cell mitogen antibody
MGC70609 antibody
MVCD1 antibody
Vascular endothelial growth factor A antibody
vascular endothelial growth factor A121 antibody
vascular endothelial growth factor A165 antibody
vascular endothelial growth factor antibody
Vascular permeability factor antibody
VEGF A antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse VEGF matched pair antibodies.<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721850" style="font-weight: bold;text-decoration: underline;">HA721850</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Mouse VEGF protein starting from 10000 pg/ml to 0 pg/ml and detect antibody [PSH02-72]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721851_1.png?v=20260908003426)
Sandwich ELISA analysis of Mouse VEGF matched pair antibodies.
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721850) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Mouse VEGF protein starting from 10000 pg/ml to 0 pg/ml and detect antibody [PSH02-72]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"