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NSUN6 Recombinant Rabbit Monoclonal Antibody [PSH02-80]

Usd: 385

Specification

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Catalog# HA721858

NSUN6 Recombinant Rabbit Monoclonal Antibody [PSH02-80]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

NSUN6 Recombinant Rabbit Monoclonal Antibody [PSH02-80]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NSUN6 aa 1-469 / 469.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 52 kDa

Positive Control

HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, rat pancreas tissue lysate.

Conjugation

unconjugated

Clone Number

PSH02-80

RRID

AB_3072970

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates.

Background References

1. Yang R et al. The RNA methyltransferase NSUN6 suppresses pancreatic cancer development by regulating cell proliferation. EBioMedicine. 2021 Jan

2. Selmi T et al. Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6. Nucleic Acids Res. 2021 Jan

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q8TEA1 Human

SwissProt: Q7TS68 Mouse

Entrez Gene: 307148 Rat

Synonyms

4933414E04Rik antibody

FLJ23743 antibody

NOL1/NOP2/Sun and PUA domain-containing protein 1 antibody

NOL1/NOP2/Sun domain family 6 antibody

NOL1/NOP2/Sun domain family member 6 antibody

NOP2 antibody

NOPD1 antibody

NSUN6 antibody

NSUN6_HUMAN antibody

nucleolar protein (NOL1/NOP2/sun) and PUA domains 1 antibody

Expand

Images

  • Western blot analysis of NSUN6 on different lysates with Rabbit anti-NSUN6 antibody (<a href="/products/HA721858" style="font-weight: bold;text-decoration: underline;">HA721858</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: Jurkat cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: Rat pancreas tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 52 kDa<br />Observed band size: 52 kDa<br /><br />Exposure time: 1 minute 58 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721858" style="font-weight: bold;text-decoration: underline;">HA721858</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NSUN6 on different lysates with Rabbit anti-NSUN6 antibody (HA721858) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: Rat pancreas tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

    Exposure time: 1 minute 58 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721858) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"