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xCT / SLC7A11 Recombinant Rabbit Monoclonal Antibody [JE31-93]

Usd: 385

Specification

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Catalog# HA721868

xCT / SLC7A11 Recombinant Rabbit Monoclonal Antibody [JE31-93]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

xCT / SLC7A11 Recombinant Rabbit Monoclonal Antibody [JE31-93]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human xCT aa 1-50 / 501.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 55 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, 293T cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, PC-12 cell lysates.

Conjugation

unconjugated

Clone Number

JE31-93

RRID

AB_3096828

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death.

Background References

1. Koppula P. et. al. Cystine transporter SLC7A11/xCT in cancer: ferroptosis, nutrient dependency, and cancer therapy. Protein Cell. 2021 Aug

2. Lin W. et. al. SLC7A11/xCT in cancer: biological functions and therapeutic implications. Am J Cancer Res. 2020 Oct

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q9UPY5 Human

SwissProt: Q9WTR6 Mouse

Entrez Gene: 310392 Rat

Synonyms

Amino acid transport system xc xCT antibody antibody

Amino acid transport system xc- antibody

Calcium channel blocker resistance protein CCBR1 antibody

Calcium channel blocker resistance protein CCBR1 antibody antibody

CCBR1 antibody

Cysteine/glutamate transporter antibody antibody

Cystine/glutamate transporter antibody

OTTHUMP00000164578 antibody

SLC7A11 antibody

Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody

Expand

Images

  • Western blot analysis of xCT / SLC7A11 on different lysates with Rabbit anti-xCT / SLC7A11 antibody (<a href="/products/HA721868" style="font-weight: bold;text-decoration: underline;">HA721868</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: 293T cell lysate (20 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 5: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 6: Rat brain tissue lysate (40 µg/Lane)<br />Lane 7: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 55 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 5 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721868" style="font-weight: bold;text-decoration: underline;">HA721868</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of xCT / SLC7A11 on different lysates with Rabbit anti-xCT / SLC7A11 antibody (HA721868) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: 293T cell lysate (20 µg/Lane)
    Lane 4: Mouse liver tissue lysate (40 µg/Lane)
    Lane 5: Mouse brain tissue lysate (40 µg/Lane)
    Lane 6: Rat brain tissue lysate (40 µg/Lane)
    Lane 7: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

    Exposure time: 5 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721868) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of xCT / SLC7A11 on PC-12 cell lysates with Rabbit anti-xCT / SLC7A11 antibody (<a href="/products/HA721868" style="font-weight: bold;text-decoration: underline;">HA721868</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721868" style="font-weight: bold;text-decoration: underline;">HA721868</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of xCT / SLC7A11 on PC-12 cell lysates with Rabbit anti-xCT / SLC7A11 antibody (HA721868) at 1/1,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721868) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • xCT / SLC7A11 was immunoprecipitated in 0.2mg HeLa cell lysate with <a href="/products/HA721868" style="font-weight: bold;text-decoration: underline;">HA721868</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA721868" style="font-weight: bold;text-decoration: underline;">HA721868</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA721868" style="font-weight: bold;text-decoration: underline;">HA721868</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA721868" style="font-weight: bold;text-decoration: underline;">HA721868</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 24 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    xCT / SLC7A11 was immunoprecipitated in 0.2mg HeLa cell lysate with HA721868 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721868 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA721868 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA721868 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 24 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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