CD53 Recombinant Rabbit Monoclonal Antibody [JE60-38] (HA721873)
Catalog# HA721873
Rabbit Monoclonal to CD53
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (HA721873) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Raji cell lysate
Lane 3: Ramos cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 35-42 kDa
Exposure time: 1 minute 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721873) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: Raji cell lysate
Lane 3: Ramos cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 35-42 kDa
Exposure time: 1 minute 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721873) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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