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SYVN1 Recombinant Rabbit Monoclonal Antibody [JE44-67]

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Specification

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Catalog# HA721874

SYVN1 Recombinant Rabbit Monoclonal Antibody [JE44-67]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SYVN1 Recombinant Rabbit Monoclonal Antibody [JE44-67]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SYVN1 aa 150-250.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 68 kDa

Positive Control

HEK-293 cell lysate, HepG2 cell lysate, Raji cell lysate, HCT 116 cell lysate, HeLa cell lysate, HeLa.

Conjugation

unconjugated

Clone Number

JE44-67

RRID

AB_3096818

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

Target

Function

E3 ubiquitin-protein ligase synoviolin is an enzyme that in humans is encoded by the SYVN1 gene. This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. This gene and the mitochondrial ribosomal protein L49 gene use in their respective 3' UTRs some of the same genomic sequence. Sequence analysis identified two transcript variants that encode different isoforms.

Background References

1. Tian F et al. LncRNA SNHG7/miR-34a-5p/SYVN1 axis plays a vital role in proliferation, apoptosis and autophagy in osteoarthritis. Biol Res. 2020 Feb

2. Shi Y et al. E3 ubiquitin ligase SYVN1 is a key positive regulator for GSDMD-mediated pyroptosis. Cell Death Dis. 2022 Feb

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q86TM6 Human

Synonyms

1200010C09Rik antibody

DER3 antibody

E3 ubiquitin-protein ligase synoviolin antibody

HMG coA reductase degradation 1 homolog antibody

HRD1 antibody

KIAA1810 antibody

MGC40372 antibody

OTTHUMP00000230429 antibody

OTTHUMP00000230430 antibody

OTTHUMP00000230431 antibody

Expand

Images

  • Western blot analysis of SYVN1 on different lysates with Rabbit anti-SYVN1 antibody (<a href="/products/HA721874" style="font-weight: bold;text-decoration: underline;">HA721874</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: Raji cell lysate<br />Lane 4: HCT 116 cell lysate<br />Lane 5: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 68 kDa<br />Observed band size: 68 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721874" style="font-weight: bold;text-decoration: underline;">HA721874</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SYVN1 on different lysates with Rabbit anti-SYVN1 antibody (HA721874) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Raji cell lysate
    Lane 4: HCT 116 cell lysate
    Lane 5: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721874) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling SYVN1 with Rabbit anti-SYVN1 antibody (<a href="/products/HA721874" style="font-weight: bold;text-decoration: underline;">HA721874</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SYVN1 antibody (<a href="/products/HA721874" style="font-weight: bold;text-decoration: underline;">HA721874</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of HeLa cells labeling SYVN1 with Rabbit anti-SYVN1 antibody (HA721874) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SYVN1 antibody (HA721874) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of HeLa cells labeling SYVN1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721874" style="font-weight: bold;text-decoration: underline;">HA721874</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling SYVN1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721874, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation