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GRK6 Recombinant Rabbit Monoclonal Antibody [JE63-06]

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Specification

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Catalog# HA721884

GRK6 Recombinant Rabbit Monoclonal Antibody [JE63-06]

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

GRK6 Recombinant Rabbit Monoclonal Antibody [JE63-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human GRK6 aa 71-120 / 576.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 66 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, MCF7 cell lysate, K-562 cell lysate, A549 cell lysate, 293T.

Conjugation

unconjugated

Clone Number

JE63-06

RRID

AB_3096781

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

G protein-coupled Receptor Kinase 6 (GRK6) is one of 7 members of the GRK serine/threonine kinase subfamily, which are known primarily for their role in desensitizing activated G protein-coupled receptors (GPCRs) . GRKs function by phosphorylating serine/threonine residues in activated GPCRs. Upon phosphorylation these residues serve as binding sites for β-arrestin proteins, inhibiting re-activation of GPCRs by blocking their re-association with G proteins . There is evidence that GRKs can also modulate selected non-GPCR signaling pathways . For example, GRK6 has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 , and the insulin-like growth factor signaling pathway. GRK6 may also play a role in immune system function. Investigators have found GRK6 expression is typically abundant in hematopoietic tumor cell lines, and a recent research study demonstrated that GRK6 suppression was selectively lethal for a number of myeloma tumor cell lines .

Background References

1. Mushegian A, Gurevich VV, Gurevich EV. The origin and evolution of G protein-coupled receptor kinases. PLoS One. 2012;7(3):e33806. doi: 10.1371/journal.pone.0033806. Epub 2012 Mar 19.

2. Gurevich EV, Tesmer JJ, Mushegian A, Gurevich VV. G protein-coupled receptor kinases: more than just kinases and not only for GPCRs. Pharmacol Ther. 2012 Jan;133(1):40-69.

3. Zheng H, Worrall C, Shen H, Issad T, Seregard S, Girnita A, Girnita L. Selective recruitment of G protein-coupled receptor kinases (GRKs) controls signaling of the insulin-like growth factor 1 receptor. Proc Natl Acad Sci U S A. 2012 May 1;109(18):7055-60.

Subcellular Location

Membrane.

UNIPROT

SwissProt: P43250 Human

Synonyms

FLJ32135 antibody

G protein coupled receptor kinase 6 antibody

G protein coupled receptor kinase GRK6 antibody

G protein-coupled receptor kinase 6 antibody

G protein-coupled receptor kinase GRK6 antibody

Gprk6 antibody

Grk6 antibody

GRK6_HUMAN antibody

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of GRK6 on different lysates with Rabbit anti-GRK6 antibody (<a href="/products/HA721884" style="font-weight: bold;text-decoration: underline;">HA721884</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si GRK6 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 66 kDa<br />Observed band size: 66 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721884" style="font-weight: bold;text-decoration: underline;">HA721884</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of GRK6 on different lysates with Rabbit anti-GRK6 antibody (HA721884) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si GRK6 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721884) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of GRK6 on different lysates with Rabbit anti-GRK6 antibody (<a href="/products/HA721884" style="font-weight: bold;text-decoration: underline;">HA721884</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: K-562 cell lysate<br />Lane 6: A549 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 66 kDa<br />Observed band size: 66 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721884" style="font-weight: bold;text-decoration: underline;">HA721884</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GRK6 on different lysates with Rabbit anti-GRK6 antibody (HA721884) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: K-562 cell lysate
    Lane 6: A549 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721884) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of 293T cells labeling GRK6.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721884" style="font-weight: bold;text-decoration: underline;">HA721884</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Mouse IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of 293T cells labeling GRK6.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721884, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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