CRTC3 Recombinant Rabbit Monoclonal Antibody [JE63-18]
Catalog# HA721887
CRTC3 Recombinant Rabbit Monoclonal Antibody [JE63-18]
-
WB
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
-
Green monkey
-
unconjugated
Overview
Product Name
CRTC3 Recombinant Rabbit Monoclonal Antibody [JE63-18]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CRTC3 aa 570-219 / 619.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 67 kDa
Positive Control
HeLa cell lysate, TT cell lysate, Jurkat cell lysate, HDLM-2 cell lysate, HEK-293 cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, human brain tissue, human hodgkin lymphoma tissue, human lung tissue, human tonsil tissue, TT, RAW264.7, PC-12.
Conjugation
unconjugated
Clone Number
JE63-18
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IHC-P
-
1:500
-
FC
-
1:1,000
Target
Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Background References
1. Hishiki T., Ohshima T., Ego T., Shimotohno K. BCL3 acts as a negative regulator of transcription from the human T-cell leukemia virus type 1 long terminal repeat through interactions with TORC3. J. Biol. Chem. 282:28335-28343 (2007)
2. Takemori H., Kanematsu M., Kajimura J., Hatano O., Katoh Y., Lin X.-Z., Min L., Yamazaki T., Doi J., Okamoto M. Dephosphorylation of TORC initiates expression of the StAR gene. Mol. Cell. Endocrinol. 265:196-204 (2007)
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
CREB regulated transcription coactivator 3 antibody
CREB-regulated transcription coactivator 3 antibody
CRTC 3 antibody
CRTC3 antibody
CRTC3_HUMAN antibody
FLJ21868 antibody
TORC 3 antibody
TORC-3 antibody
TORC3 antibody
Transducer of CREB protein 3 antibody
ExpandImages
-
Western blot analysis of CRTC3 on different lysates with Rabbit anti-CRTC3 antibody (HA721887) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: TT cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HDLM-2 cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 67 kDa
Observed band size: 75 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721887) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of TT cells labeling CRTC3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721887, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of RAW264.7 cells labeling CRTC3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721887, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of PC-12 cells labeling CRTC3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721887, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"