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CRTC3 Recombinant Rabbit Monoclonal Antibody [JE63-18]

Usd: 385

Specification

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Catalog# HA721887

CRTC3 Recombinant Rabbit Monoclonal Antibody [JE63-18]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

CRTC3 Recombinant Rabbit Monoclonal Antibody [JE63-18]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CRTC3 aa 570-619 / 619.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 67 kDa

Positive Control

HeLa cell lysate, TT cell lysate, Jurkat cell lysate, HDLM-2 cell lysate, HEK-293 cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, human brain tissue, human hodgkin lymphoma tissue, human lung tissue, human tonsil tissue, TT, RAW264.7, PC-12.

Conjugation

unconjugated

Clone Number

JE63-18

RRID

AB_3096768

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:500

  • FC

  • 1:1,000

Target

Function

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

Background References

1. Hishiki T., Ohshima T., Ego T., Shimotohno K. BCL3 acts as a negative regulator of transcription from the human T-cell leukemia virus type 1 long terminal repeat through interactions with TORC3. J. Biol. Chem. 282:28335-28343 (2007)

2. Takemori H., Kanematsu M., Kajimura J., Hatano O., Katoh Y., Lin X.-Z., Min L., Yamazaki T., Doi J., Okamoto M. Dephosphorylation of TORC initiates expression of the StAR gene. Mol. Cell. Endocrinol. 265:196-204 (2007)

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q6UUV7 Human

SwissProt: Q91X84 Mouse

Entrez Gene: 365297 Rat

Synonyms

CREB regulated transcription coactivator 3 antibody

CREB-regulated transcription coactivator 3 antibody

CRTC 3 antibody

CRTC3 antibody

CRTC3_HUMAN antibody

FLJ21868 antibody

TORC 3 antibody

TORC-3 antibody

TORC3 antibody

Transducer of CREB protein 3 antibody

Expand

Images

  • Western blot analysis of CRTC3 on different lysates with Rabbit anti-CRTC3 antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: TT cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: HDLM-2 cell lysate<br />Lane 5: HEK-293 cell lysate<br />Lane 6: COS-1 cell lysate<br />Lane 7: RAW264.7 cell lysate<br />Lane 8: NIH/3T3 cell lysate<br />Lane 9: PC-12 cell lysate<br />Lane 10: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 67 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CRTC3 on different lysates with Rabbit anti-CRTC3 antibody (HA721887) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: TT cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: HDLM-2 cell lysate
    Lane 5: HEK-293 cell lysate
    Lane 6: COS-1 cell lysate
    Lane 7: RAW264.7 cell lysate
    Lane 8: NIH/3T3 cell lysate
    Lane 9: PC-12 cell lysate
    Lane 10: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 67 kDa
    Observed band size: 75 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721887) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CRTC3 antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Rabbit anti-CRTC3 antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CRTC3 antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CRTC3 antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of TT cells labeling CRTC3.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of TT cells labeling CRTC3.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721887, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of RAW264.7 cells labeling CRTC3.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of RAW264.7 cells labeling CRTC3.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721887, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of PC-12 cells labeling CRTC3.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721887" style="font-weight: bold;text-decoration: underline;">HA721887</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PC-12 cells labeling CRTC3.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721887, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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