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RAC3 Recombinant Rabbit Monoclonal Antibody [JE56-48]

Usd: 385

Specification

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Catalog# HA721890

RAC3 Recombinant Rabbit Monoclonal Antibody [JE56-48]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

RAC3 Recombinant Rabbit Monoclonal Antibody [JE56-48]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human RAC3 aa 71-170 / 192.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 21 kDa

Positive Control

HEK-293 cell lysate, JAR cell lysate, HUVEC cell lysate, PC-12 cell lysate, SH-SY5Y cell lysate, C2C12 cell lysate.

Conjugation

unconjugated

Clone Number

JE56-48

RRID

AB_3096678

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

Target

Function

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.

Background References

1. Haataja L., Kaartinen V., Groffen J., Heisterkamp N. The small GTPase Rac3 interacts with the integrin-binding protein CIB and promotes integrin alpha(IIb)beta(3)-mediated adhesion and spreading. J. Biol. Chem. 277:8321-8328 (2002)

2. De Langhe S., Haataja L., Senadheera D., Groffen J., Heisterkamp N. Interaction of the small GTPase Rac3 with NRBP, a protein with a kinase- homology domain. Int. J. Mol. Med. 9:451-459 (2002)

Subcellular Location

Cytoplasm, Endomembrane system, Cell projection, lamellipodium, Cytoplasm, perinuclear region, Cell membrane, cytoskeleton.

UNIPROT

SwissProt: P60763 Human

SwissProt: P60764 Mouse

Synonyms

OTTMUSP00000004488 antibody

p21 Rac3 antibody

p21-Rac3 antibody

Rac1B antibody

RAC3 antibody

RAC3_HUMAN antibody

RAS related C3 botulinum substrate 3 antibody

Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) antibody

Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) antibody

Ras-related C3 botulinum toxin substrate 3 antibody

Expand

Images

  • Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (<a href="/products/HA721890" style="font-weight: bold;text-decoration: underline;">HA721890</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (20 µg/Lane)<br />Lane 2: JAR cell lysate (20 µg/Lane)<br />Lane 3: HUVEC cell lysate (20 µg/Lane)<br />Lane 4: PC-12 cell lysate (20 µg/Lane)<br />Lane 5: SH-SY5Y cell lysate (15 µg/Lane)<br />Lane 6: C2C12 cell lysate (15 µg/Lane)<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721890" style="font-weight: bold;text-decoration: underline;">HA721890</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (HA721890) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate (20 µg/Lane)
    Lane 2: JAR cell lysate (20 µg/Lane)
    Lane 3: HUVEC cell lysate (20 µg/Lane)
    Lane 4: PC-12 cell lysate (20 µg/Lane)
    Lane 5: SH-SY5Y cell lysate (15 µg/Lane)
    Lane 6: C2C12 cell lysate (15 µg/Lane)

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721890) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (<a href="/products/HA721890" style="font-weight: bold;text-decoration: underline;">HA721890</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-RAC3 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721890" style="font-weight: bold;text-decoration: underline;">HA721890</a>) at 1/5,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (HA721890) at 1/5,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-RAC3 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721890) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation