ICAM2 Recombinant Rabbit Monoclonal Antibody [JE63-28]
Catalog# HA721893
ICAM2 Recombinant Rabbit Monoclonal Antibody [JE63-28]
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WB
-
IHC-P
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Mouse
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Rat
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unconjugated
Overview
Product Name
ICAM2 Recombinant Rabbit Monoclonal Antibody [JE63-28]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse ICAM2 aa 1-250 / 277.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 31 kDa
Positive Control
Mouse spleen tissue lysate, mouse heart tissue lysate, mouse kidney tissue lysate, rat spleen tissue lysate, mouse spleen tissue, rat spleen tissue, rat lung tissue.
Conjugation
unconjugated
Clone Number
JE63-28
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:500
Target
Function
The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
Background References
1. Xu G., Shin S.B., Jaffrey S.R. Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini. Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315 (2009)
2. Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. J. Proteome Res. 4:2070-2080 (2005)
Subcellular Location
Membrane.
UNIPROT
Synonyms
CD102 antibody
CD102 antigen antibody
ICAM 2 antibody
ICAM-2 antibody
Icam2 antibody
ICAM2_HUMAN antibody
Intercellular adhesion molecule 2 antibody
Ly60 antibody
Images
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Western blot analysis of ICAM2 on different lysates with Rabbit anti-ICAM2 antibody (HA721893) at 1/1,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse heart tissue lysate (no heat)
Lane 3: Mouse kidney tissue lysate (no heat)
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse spleen tissue lysate (no heat)
Lane 6: Rat spleen tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 45-50 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721893) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ICAM2 antibody (HA721893) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721893) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-ICAM2 antibody (HA721893) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721893) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-ICAM2 antibody (HA721893) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721893) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"