Lhx2 Recombinant Rabbit Monoclonal Antibody [JE54-99]
Catalog# HA721900
Lhx2 Recombinant Rabbit Monoclonal Antibody [JE54-99]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Lhx2 Recombinant Rabbit Monoclonal Antibody [JE54-99]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Lhx2 aa 1-100 / 406.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 44 kDa
Positive Control
A431 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE54-99
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.
Background References
1. Xie T., Du K., Liu W., Liu C., Wang B., Tian Y., Li R., Huang X., Lin J., Yuan Y. LHX2 facilitates the progression of nasopharyngeal carcinoma via activation of the FGF1/FGFR axis. Br J Cancer 127:1239-1253 (2022).
2. Durante M.A., Kurtenbach S., Sargi Z.B., Harbour J.W., Choi R., Kurtenbach S., Goss G.M., Matsunami H., Goldstein B.J. Single-cell analysis of olfactory neurogenesis and differentiation in adult humans. Nat Neurosci 23:323-326 (2020).
Subcellular Location
Nucleus.
Synonyms
hLhx2 antibody
Homeobox protein LH-2 antibody
Homeobox protein LH2 antibody
LH2 antibody
LHX2 antibody
LHX2_HUMAN antibody
LIM homeobox 2 antibody
LIM homeobox protein 2 antibody
LIM HOX gene 2 antibody
LIM/homeobox protein Lhx2 antibody
Images
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Western blot analysis of Lhx2 on different lysates with Rabbit anti-Lhx2 antibody (HA721900) at 1/1,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 40-44 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721900) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"