T-bet / Tbx21 Recombinant Rabbit Monoclonal Antibody [JE60-20]
Catalog# HA721901
T-bet / Tbx21 Recombinant Rabbit Monoclonal Antibody [JE60-20]
-
WB
-
IHC-P
-
IF-Cell
-
FC
-
Human
-
unconjugated
Overview
Product Name
T-bet / Tbx21 Recombinant Rabbit Monoclonal Antibody [JE60-20]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Tbx21 aa 21-70 / 535.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell, FC
Molecular Weight
Predicted band size: 58 kDa
Positive Control
NK-92 cell lysate, human hodgkin's lymphoma tissue, human thymus tissue, human tonsil tissue, NK-92.
Conjugation
unconjugated
Clone Number
JE60-20
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:200
-
IF-Cell
-
1:100
-
FC
-
1:1,000
Target
Function
T-box transcription factor TBX21, also called T-bet (T-box expressed in T cells), is a protein that in humans is encoded by the TBX21 gene. Though being for long thought of only as a master regulator of type 1 immune response, T-bet has recently been shown to be implicated in development of various immune cell subsets and maintenance of mucosal homeostasis. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNg). Expression of the human ortholog also correlates with IFNg expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
Background References
1. Yang X et al. TBX21 inhibits colorectal cancer metastasis through ARHGAP29/GSK3beta inhibitory signaling- and MYCT1/ZO-1 signaling-dependent manner. Int J Biol Sci. 2025 Jan
2. Zou Z et al. TBX21 knockdown attenuates neuroinflammation induced by intracerebral hemorrhage via the SIRT1-WDR5-H3K4me3 axis. Brain Res Bull. 2025 Sep
Subcellular Location
Nucleus.
UNIPROT
Synonyms
T bet antibody
T box 21 antibody
T box expressed in T cells antibody
T box protein 21 antibody
T box transcription factor TBX21 antibody
T cell specific T box transcription factor antibody
T cell specific T box transcription factor T bet antibody
T PET antibody
T-box protein 21 antibody
T-box transcription factor TBX21 antibody
ExpandImages
-
☑ Relative expression (RE)
Western blot analysis of T-bet / Tbx21 on different lysates with Rabbit anti-T-bet / Tbx21 antibody (HA721901) at 1/1,000 dilution.
Lane 1: NK-92 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (negative) (20 µg/Lane)
Lane 3: Jurkat cell lysate (negative) (20 µg/Lane)
Predicted band size: 58 kDa
Observed band size: 65 kDa
Exposure time: 5 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721901) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue with Rabbit anti-T-bet / Tbx21 antibody (HA721901) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-T-bet / Tbx21 antibody (HA721901) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-T-bet / Tbx21 antibody (HA721901) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunocytochemistry analysis of NK-92 (positive) and K-562 (negative) labeling T-bet / Tbx21 with Rabbit anti-T-bet / Tbx21 antibody (HA721901) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-T-bet / Tbx21 antibody (HA721901) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of NK-92 cells labeling T-bet / Tbx21.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721901, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"