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ABHD5 Recombinant Rabbit Monoclonal Antibody [JE60-62]

Usd: 385

Specification

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Catalog# HA721903

ABHD5 Recombinant Rabbit Monoclonal Antibody [JE60-62]

Application

  • WB

  • FC

Reactivity

  • Mouse

Predicted reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ABHD5 Recombinant Rabbit Monoclonal Antibody [JE60-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ABHD5 aa 151-200 / 349.

Species Reactivity

Mouse (Predicted: Human)

Predicted species support after-sales service

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 39 kDa

Positive Control

NIH/3T3 cell lysate, mouse pancreas tissue lysate, mouse kidney tissue lysate, NIH/3T3.

Conjugation

unconjugated

Clone Number

JE60-62

RRID

AB_3096734

Product Features

Form

Liquid

Concentration

1 mg/ml(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors. Functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.

Background References

1. Ghosh A.K., Ramakrishnan G., Chandramohan C., Rajasekharan R. CGI-58, the causative gene for Chanarin-Dorfman syndrome, mediates acylation of lysophosphatidic acid. J. Biol. Chem. 283:24525-24533.

2. Lass A., Zimmermann R., Haemmerle G., Riederer M., Schoiswohl G., Schweiger M., Kienesberger P., Strauss J.G., Gorkiewicz G., Zechner R. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome. Cell Metab. 3:309-319 (2006).

3. Akiyama M., Sakai K., Takayama C., Yanagi T., Yamanaka Y., McMillan J.R., Shimizu H. CGI-58 is an alpha/beta-hydrolase within lipid transporting lamellar granules of differentiated keratinocytes. Am. J. Pathol. 173:1349-1360 (2008).

Subcellular Location

Cytoplasm, Lipid droplet.

UNIPROT

SwissProt: Q8WTS1 Human

SwissProt: Q9DBL9 Mouse

Synonyms

1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody

ABHD5 antibody

ABHD5_HUMAN antibody

Abhydrolase domain containing 5 antibody

Abhydrolase domain containing protein 5 antibody

Abhydrolase domain-containing protein 5 antibody

CDS antibody

CGI 58 antibody

CGI58 antibody

CGI58 protein antibody

Expand

Images

  • Western blot analysis of ABHD5 on different lysates with Rabbit anti-ABHD5 antibody (<a href="/products/HA721903" style="font-weight: bold;text-decoration: underline;">HA721903</a>) at 1/2,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 2: Mouse pancreas tissue lysate (40 µg/Lane)<br />Lane 3: Mouse kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 39 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721903" style="font-weight: bold;text-decoration: underline;">HA721903</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ABHD5 on different lysates with Rabbit anti-ABHD5 antibody (HA721903) at 1/2,000 dilution.

    Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 2: Mouse pancreas tissue lysate (40 µg/Lane)
    Lane 3: Mouse kidney tissue lysate (40 µg/Lane)

    Predicted band size: 39 kDa
    Observed band size: 43 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721903) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of NIH/3T3 cells labeling ABHD5.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721903" style="font-weight: bold;text-decoration: underline;">HA721903</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of NIH/3T3 cells labeling ABHD5.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721903, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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