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ATAD2 Recombinant Rabbit Monoclonal Antibody [JE34-52]

Usd: 385

Specification

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Catalog# HA721905

ATAD2 Recombinant Rabbit Monoclonal Antibody [JE34-52]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ATAD2 Recombinant Rabbit Monoclonal Antibody [JE34-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human ATAD2 aa 31-130 / 1,390.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 158 kDa

Positive Control

HeLa cell lysate, MCF7 cell lysate, T-47D cell lysate, HT-29 cell lysate, Saos- 2 cell lysate, HepG2 cell lysate, Jurkat cell lysate, human testis tissue.

Conjugation

unconjugated

Clone Number

JE34-52

RRID

AB_3096529

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:2,000

Target

Function

May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.

Background References

1. Zou J.X., Revenko A.S., Li L.B., Gemo A.T., Chen H.-W. ANCCA, an estrogen-regulated AAA+ ATPase coactivator for ERalpha, is required for coregulator occupancy and chromatin modification. Proc. Natl. Acad. Sci. U.S.A. 104:18067-18072 (2007)。

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q6PL18 Human

Synonyms

AAA nuclear coregulator cancer-associated protein antibody

AAA nuclear coregulator, cancer-associated antibody

ANCCA antibody

Atad2 antibody

ATAD2_HUMAN antibody

ATPase family AAA domain containing 2 antibody

ATPase family AAA domain containing protein 2 antibody

ATPase family AAA domain-containing protein 2 antibody

CT137 antibody

DKFZp667N1320 antibody

Expand

Images

  • Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody (<a href="/products/HA721905" style="font-weight: bold;text-decoration: underline;">HA721905</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: MCF7 cell lysate<br />Lane 3: T-47D cell lysate<br />Lane 4: HT-29 cell lysate<br />Lane 5: Saos- 2 cell lysate<br />Lane 6: HepG2 cell lysate<br />Lane 7: Jurkat cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 158 kDa<br />Observed band size: 180 kDa<br /><br />Exposure time: 4 minutes 41 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721905" style="font-weight: bold;text-decoration: underline;">HA721905</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody (HA721905) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: T-47D cell lysate
    Lane 4: HT-29 cell lysate
    Lane 5: Saos- 2 cell lysate
    Lane 6: HepG2 cell lysate
    Lane 7: Jurkat cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 158 kDa
    Observed band size: 180 kDa

    Exposure time: 4 minutes 41 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721905) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody (<a href="/products/HA721905" style="font-weight: bold;text-decoration: underline;">HA721905</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-WT cell lysate<br />Lane 2: A549-KD ATAD2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 158 kDa<br />Observed band size: 180 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721905" style="font-weight: bold;text-decoration: underline;">HA721905</a>) at 1/1,000 dilution was used in primary antibody diluent at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody (HA721905) at 1/1,000 dilution.

    Lane 1: A549-WT cell lysate
    Lane 2: A549-KD ATAD2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 158 kDa
    Observed band size: 180 kDa

    Exposure time: 1 minute; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721905) at 1/1,000 dilution was used in primary antibody diluent at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATAD2 antibody (<a href="/products/HA721905" style="font-weight: bold;text-decoration: underline;">HA721905</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721905" style="font-weight: bold;text-decoration: underline;">HA721905</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATAD2 antibody (HA721905) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721905) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"