ATAD2 Recombinant Rabbit Monoclonal Antibody [JE34-52] (HA721905)
Catalog# HA721905
Rabbit Monoclonal to ATAD2
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody (HA721905) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: T-47D cell lysate
Lane 4: HT-29 cell lysate
Lane 5: Saos- 2 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 158 kDa
Observed band size: 180 kDa
Exposure time: 4 minutes 41 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721905) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: T-47D cell lysate
Lane 4: HT-29 cell lysate
Lane 5: Saos- 2 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 158 kDa
Observed band size: 180 kDa
Exposure time: 4 minutes 41 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721905) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.