Lrp2 / Megalin Recombinant Rabbit Monoclonal Antibody [PSH02-91]
Catalog# HA721910
Lrp2 / Megalin Recombinant Rabbit Monoclonal Antibody [PSH02-91]
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IHC-P
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IF-Tissue
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WB
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Human
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unconjugated
Overview
Product Name
Lrp2 / Megalin Recombinant Rabbit Monoclonal Antibody [PSH02-91]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Lrp2 aa 2,500-3,200 / 4,655.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue, WB
Target Molecular Weight
Predicted band size: 522 kDa
Positive Control
Human kidney tissue lysates, human kidney tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
PSH02-91
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000
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IF-Tissue
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1:50
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WB
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1:2,000
Target
Function
LRP2 was identified as the antigen of rat experimental membranous nephropathy (Heyman nephritis) and originally named gp330 and subsequently megalin and later LRP2. LRP2/megalin is a multiligand binding receptor found in the plasma membrane of many absorptive epithelial cells. LRP2 is an approximately 600kDa (4665 amino acids) transmembrane glycoprotein with structural similarities to the low density lipoprotein receptor (LDLR). LRP2 has a NPXY motif that is the binding site for Dab2 to initiate clathrin-mediated endocytosis. LRP2 forms a homodimer that changes conformation in response to pH. At pH 7.5 (extracellular pH), LRP2 is considered active, with the leucine loops in an open conformation to allow ligands to bind.[11] At acidic endosomal pHs, the leucine loops collapse to prevent ligands binding.
Background References
1. Beenken A et al. Structures of LRP2 reveal a molecular machine for endocytosis. Cell. 2023 Feb
2. Faridi R et al. Variants of LRP2, encoding a multifunctional cell-surface endocytic receptor, associated with hearing loss and retinal dystrophy. Clin Genet. 2023 Jun
Subcellular Location
Apical cell membrane, Endosome lumen, Membrane, coated pit, Cell projection, dendrite, axon.
UNIPROT
Synonyms
Calcium sensor protein antibody
DBS antibody
Glycoprotein 330 antibody
gp330 antibody
Heymann nephritis antigen homolog antibody
Low-density lipoprotein receptor-related protein 2 antibody
LRP-2 antibody
Lrp2 antibody
LRP2_HUMAN antibody
Megalin antibody
Images
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Western blot analysis of Lrp2 / Megalin on human kidney tissue lysates with Rabbit anti-Lrp2 / Megalin antibody (HA721910) at 1/2,000 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 522 kDa
Observed band size: 600 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721910) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Lrp2 / Megalin antibody (HA721910) at 1/1000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721910) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-Lrp2 / Megalin antibody (HA721910) at 1/1000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721910) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Lrp2 / Megalin with Rabbit anti-Lrp2 / Megalin antibody (HA721910) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721910, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Lrp2 / Megalin Recombinant Rabbit Monoclonal Antibody [PSH02-91] - BSA and Azide free
Application: IHC-P,IF-Tissue,WB
Reactivity: Human
Conjugate: unconjugated
Lrp2 / Megalin Recombinant Rabbit Monoclonal Antibody [PSH04-52]
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
