HLA Class II DRB1 Recombinant Rabbit Monoclonal Antibody [JE60-43]
Catalog# HA721927
HLA Class II DRB1 Recombinant Rabbit Monoclonal Antibody [JE60-43]
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WB
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
HLA Class II DRB1 Recombinant Rabbit Monoclonal Antibody [JE60-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human HLA Class II DRB1 aa 151-200 / 266.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 30 kDa
Positive Control
Ramos cell lysate, Raji cell lysate, Daudi cell lysate, HDLM-2 cell lysate, A375 cell lysate, human tonsil tissue, human skin tissue, human diffuse large B-cell lymphoma tissue.
Conjugation
unconjugated
Clone Number
JE60-43
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
-
1:1,000
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IF-Tissue
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1:100
Target
Function
A beta chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the alpha chain HLA-DRA, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DRB1-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides. The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules.
Background References
1. Kwok W.W., Tan V., Gillette L., Littell C.T., Soltis M.A., LaFond R.B., Yang J., James E.A., DeLong J.H. Frequency of epitope-specific naive CD4(+) T cells correlates with immunodominance in the human memory repertoire. J. Immunol. 188:2537-2544 (2012).
2. Abelin J.G., Harjanto D., Malloy M., Suri P., Colson T., Goulding S.P., Creech A.L., Serrano L.R., Nasir G. Defining HLA-II Ligand Processing and Binding Rules with Mass Spectrometry Enhances Cancer Epitope Prediction. Immunity 0:0-0 (2019).
Subcellular Location
Cell membrane. Endoplasmic reticulum.
UNIPROT
Synonyms
2B1F_HUMAN antibody
DR1 antibody
DR16 antibody
DR5 antibody
DR8 antibody
DRB1 antibody
DRw10 antibody
DW2.2/DR2.2 antibody
FLJ75017 antibody
FLJ76359 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of HLA Class II DRB1 on different lysates with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Raji cell lysate
Lane 3: Daudi cell lysate
Lane 4: HDLM-2 cell lysate
Lane 5: A375 cell lysate
Lane 6: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721927) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721927) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721927) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721927) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling HLA Class II DRB1 with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721927, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling HLA Class II DRB1 with Rabbit anti-HLA Class II DRB1 antibody (HA721927) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721927, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"