PHD3 Recombinant Rabbit Monoclonal Antibody [JE60-82]
Catalog# HA721929
PHD3 Recombinant Rabbit Monoclonal Antibody [JE60-82]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
PHD3 Recombinant Rabbit Monoclonal Antibody [JE60-82]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant full length protein within human PHD3.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 27 kDa
Positive Control
Mouse liver tissue lysate, K-562 cell lysate, A375 cell lysate, HEK-293 cell lysate.
Conjugation
unconjugated
Clone Number
JE60-82
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism.
Background References
1. Xie L., Xiao K., Whalen E.J., Forrester M.T., Freeman R.S., Fong G., Gygi S.P., Lefkowitz R.J., Stamler J.S. Oxygen-regulated beta(2)-adrenergic receptor hydroxylation by EGLN3 and ubiquitylation by pVHL. Sci. Signal. 2:RA33-RA33 (2009).
2. Luo W., Hu H., Chang R., Zhong J., Knabel M., O\'Meally R., Cole R.N., Pandey A., Semenza G.L. Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1. Cell 145:732-744 (2011).
Subcellular Location
Cytoplasm. Nucleus.
Synonyms
Egl 9 family hypoxia inducible factor 3 antibody
Egl nine homolog 3 (C. elegans) antibody
Egl nine homolog 3 antibody
Egl nine like protein 3 isoform antibody
EGL9 homolog of C. elegans 3 antibody
EGLN3 antibody
EGLN3_HUMAN antibody
Factor responsive smooth muscle protein antibody
HIF Prolyl Hydroxylase 3 antibody
HIF-PH3 antibody
ExpandImages
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Western blot analysis of PHD3 on different lysates with Rabbit anti-PHD3 antibody (HA721929) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate (40 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: A375 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Predicted band size: 27 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"