CD32A + CD32B + CD32C Recombinant Rabbit Monoclonal Antibody [JE64-17]
Catalog# HA721930
CD32A + CD32B + CD32C Recombinant Rabbit Monoclonal Antibody [JE64-17]
-
WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
CD32A + CD32B + CD32C Recombinant Rabbit Monoclonal Antibody [JE64-17]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CD32A aa 60-109 / 317.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 35 kDa
Positive Control
K-562 cell lysate, Raji cell lysate, HL-60 cell lysate, human lung tissue lysate, human pancreas tissue, human spleen tissue, human tonsil tissue.
Conjugation
unconjugated
Clone Number
JE64-17
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:500-1:2,000
Target
Function
CD32A: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. CD32B: Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis. CD32C: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells.
Background References
1. Lu J., Marnell L.L., Marjon K.D., Mold C., Du Clos T.W., Sun P.D. Structural recognition and functional activation of FcgammaR by innate pentraxins. Nature 456:989-992 (2008).
Subcellular Location
Cell membrane.
Synonyms
Low affinity immunoglobulin gamma Fc region receptor II-a
Low affinity immunoglobulin gamma Fc region receptor II-b
Low affinity immunoglobulin gamma Fc region receptor II-c
IgG Fc receptor II-a
IgG Fc receptor II-b
IgG Fc receptor II-c
CDw32
Fc-gamma RII-a (Fc-gamma-RIIa
FcRII-a)
Fc-gamma RII-b (Fc-gamma-RIIb
ExpandImages
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Western blot analysis of CD32A + CD32B + CD32C on different lysates with Rabbit anti-CD32A + CD32B + CD32C antibody (HA721930) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: Raji cell lysate (20 µg/Lane)
Lane 3: HL-60 cell lysate (20 µg/Lane)
Lane 4: Human lung tissue lysate (30 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 35-40 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721930) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-CD32A + CD32B + CD32C antibody (HA721930) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721930) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD32A + CD32B + CD32C antibody (HA721930) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721930) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD32A + CD32B + CD32C antibody (HA721930) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721930) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"