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BNP Recombinant Rabbit Monoclonal Antibody [JE30-94]

Usd: 385

Specification

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Catalog# HA721946

BNP Recombinant Rabbit Monoclonal Antibody [JE30-94]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

BNP Recombinant Rabbit Monoclonal Antibody [JE30-94]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human BNP aa 61-110 / 134.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 15 kDa

Positive Control

SW480 cell lysate, U-87 MG cell lysate, U-2 OS cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE30-94

RRID

AB_3096752

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The presence of myocardial injury is a significant predictor of mortality in hospitalized coronavirus disease 2019 (COVID-19) patients, and there is evidence of increased levels of natriuretic peptide B in hospitalized non-survivor COVID-19 patients. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis.

Background References

1. Nishikimi T et al. Potential pitfalls when interpreting plasma BNP levels in heart failure practice. J Cardiol. 2021 Oct

2. Rørth R et al. Comparison of BNP and NT-proBNP in Patients With Heart Failure and Reduced Ejection Fraction. Circ Heart Fail. 2020 Feb

Subcellular Location

Secreted.

UNIPROT

SwissProt: P16860 Human

SwissProt: P40753 Mouse

SwissProt: P13205 Rat

Synonyms

BNP(1-28) antibody

BNP(1-29) antibody

BNP(1-30) antibody

BNP(1-32) antibody

BNP(2-31) antibody

BNP(3-29) antibody

BNP(3-30) antibody

BNP(3-32) antibody

BNP(4-27) antibody

BNP(4-29) antibody

Expand

Images

  • Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (<a href="/products/HA721946" style="font-weight: bold;text-decoration: underline;">HA721946</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW480 cell lysate (20 µg/Lane)<br />Lane 2: U-87 MG cell lysate (20 µg/Lane)<br />Lane 3: U-2 OS cell lysate (20 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (30 µg/Lane)<br />Lane 5: Rat brain tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 15 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721946" style="font-weight: bold;text-decoration: underline;">HA721946</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (HA721946) at 1/1,000 dilution.

    Lane 1: SW480 cell lysate (20 µg/Lane)
    Lane 2: U-87 MG cell lysate (20 µg/Lane)
    Lane 3: U-2 OS cell lysate (20 µg/Lane)
    Lane 4: Mouse brain tissue lysate (30 µg/Lane)
    Lane 5: Rat brain tissue lysate (30 µg/Lane)

    Predicted band size: 15 kDa
    Observed band size: 37 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation