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Mouse IFN gamma Recombinant Rabbit Monoclonal Antibody [PSH03-20] - BSA and Azide free (Capture)

Usd: 649

Specification

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Catalog# HA721956

Mouse IFN gamma Recombinant Rabbit Monoclonal Antibody [PSH03-20] - BSA and Azide free (Capture)

Application

  • ELISA(Cap)

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Mouse IFN gamma Recombinant Rabbit Monoclonal Antibody [PSH03-20] - BSA and Azide free (Capture)

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Mouse IFN-gamma aa 23-155.

Species Reactivity

Mouse

Validated Applications

ELISA(Cap)

Conjugation

unconjugated

Clone Number

PSH03-20

RRID

AB_3096708

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA(Cap)

  • 1:1,000

Target

Function

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Background References

1. Liu W et al. IFN-gamma, should not be ignored in SLE. Front Immunol. 2022 Aug

2. Clark DN et al. Unique aspects of IFN-gamma/STAT1 signaling in neurons. Immunol Rev. 2022 Oct

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01580 Mouse

Synonyms

IF 1 antibody

IFG antibody

IFI antibody

ifn g antibody

IFN gamma antibody

IFN immune antibody

IFN, immune antibody

IFN-gamma antibody

IFNG antibody

IFNG_HUMAN antibody

Expand

Images

  • Sandwich ELISA analysis of Mouse IFN gamma matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721956" style="font-weight: bold;text-decoration: underline;">HA721956</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Mouse IFN gamma protein starting from 2000 pg/ml to 0 pg/ml and detect antibody-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of Mouse IFN gamma matched pair antibodies

    Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721956) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Mouse IFN gamma protein starting from 2000 pg/ml to 0 pg/ml and detect antibody-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"