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TRPM8 Recombinant Rabbit Monoclonal Antibody [JE32-58]

Usd: 385

Specification

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Catalog# HA721959

TRPM8 Recombinant Rabbit Monoclonal Antibody [JE32-58]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

TRPM8 Recombinant Rabbit Monoclonal Antibody [JE32-58]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TRPM8 aa 851-1,050 / 1,104.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 128 kDa

Positive Control

LNCaP cell lysate, MCF7 cell lysate, HCT 116 cell lysate, human liver cancer tissue, human prostate cancer tissue.

Conjugation

unconjugated

Clone Number

JE32-58

RRID

AB_3096699

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:500

Target

Function

Transient receptor potential cation channel subfamily M (melastatin) member 8 (TRPM8), also known as the cold and menthol receptor 1 (CMR1), is a protein that in humans is encoded by the TRPM8 gene. The TRPM8 channel is the primary molecular transducer of cold somatosensation in humans. In addition, mints can desensitize a region through the activation of TRPM8 receptors (the 'cold'/menthol receptor). TRPM8 is an ion channel: upon activation, it allows the entry of Na+ and Ca2+ ions into the cell, which leads to depolarization and the generation of an action potential. The signal is conducted from primary afferents (type C- and A-delta) eventually leading to the sensation of cold and cold pain. The TRPM8 protein is expressed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin whereas WS-12 and CPS-369 are the most selective agonists of TRPM8. TRPM8 is also expressed in the prostate, lungs, and bladder where its function is not well understood.

Background References

1. Yin Y et al. Activation mechanism of the mouse cold-sensing TRPM8 channel by cooling agonist and PIP2. Science. 2022 Oct

2. Izquierdo C et al. TRPM8 Channels: Advances in Structural Studies and Pharmacological Modulation. Int J Mol Sci. 2021 Aug

Subcellular Location

Cell membrane, Membrane raft, Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q7Z2W7 Human

Synonyms

Long transient receptor potential channel 6 antibody

LTrpC-6 antibody

LTrpC6 antibody

MGC2849 antibody

Short form of the TRPM8 cationic channel antibody

Transient receptor potential cation channel subfamily M member 8 antibody

Transient receptor potential p8 antibody

transient receptor potential-p8 antibody

Trp p8 antibody

Trp-p8 antibody

Expand

Images

  • Western blot analysis of TRPM8 on different lysates with Rabbit anti-TRPM8 antibody (<a href="/products/HA721959" style="font-weight: bold;text-decoration: underline;">HA721959</a>) at 1/1,000 dilution.<br /><br />Lane 1: LNCaP cell lysate<br />Lane 2: MCF7 cell lysate<br />Lane 3: HCT 116 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 128 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721959" style="font-weight: bold;text-decoration: underline;">HA721959</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TRPM8 on different lysates with Rabbit anti-TRPM8 antibody (HA721959) at 1/1,000 dilution.

    Lane 1: LNCaP cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: HCT 116 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 128 kDa
    Observed band size: 110 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721959) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-TRPM8 antibody (<a href="/products/HA721959" style="font-weight: bold;text-decoration: underline;">HA721959</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721959" style="font-weight: bold;text-decoration: underline;">HA721959</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-TRPM8 antibody (HA721959) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721959) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-TRPM8 antibody (<a href="/products/HA721959" style="font-weight: bold;text-decoration: underline;">HA721959</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721959" style="font-weight: bold;text-decoration: underline;">HA721959</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-TRPM8 antibody (HA721959) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721959) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"