p18 INK4c Recombinant Rabbit Monoclonal Antibody [JE36-09]
Catalog# HA721960
p18 INK4c Recombinant Rabbit Monoclonal Antibody [JE36-09]
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WB
-
IHC-P
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
p18 INK4c Recombinant Rabbit Monoclonal Antibody [JE36-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human p18 INK4c aa 1-150 / 168.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 18 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, A549 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human skeletal muscle tissue.
Conjugation
unconjugated
Clone Number
JE36-09
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:500
Target
Function
Cyclin-dependent kinase 4 inhibitor C is an enzyme that in humans is encoded by the CDKN2C gene. The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported.
Background References
1. Schmalzbauer BS et al. CDK6 Degradation Is Counteracted by p16INK4A and p18INK4C in AML. Cancers (Basel). 2022 Mar
Subcellular Location
Cytoplasm, cytosol, nucleus.
Synonyms
CDK6 inhibitor p18 antibody
CDKN 2C antibody
CDKN 6 antibody
Cdkn2c antibody
CDKN6 antibody
CDN2C_HUMAN antibody
Cyclin dependent inhibitor antibody
Cyclin dependent kinase 4 inhibitor C antibody
Cyclin dependent kinase 6 inhibitor antibody
Cyclin dependent kinase 6 inhibitor p18 antibody
ExpandImages
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Western blot analysis of p18 INK4c on different lysates with Rabbit anti-p18 INK4c antibody (HA721960) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 6: Rat skeletal muscle tissue lysate (40 µg/Lane)
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721960) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-p18 INK4c antibody (HA721960) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721960) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"