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KSR1 Recombinant Rabbit Monoclonal Antibody [JE57-75]

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Specification

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Catalog# HA721963

KSR1 Recombinant Rabbit Monoclonal Antibody [JE57-75]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

KSR1 Recombinant Rabbit Monoclonal Antibody [JE57-75]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human KSR1 aa 874-923 / 923.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 102 kDa

Positive Control

HeLa cell lysate, HDLM-2 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, C2C12 cell lysate, C6 cell lysate, human cervical carcinoma tissue, C6.

Conjugation

unconjugated

Clone Number

JE57-75

RRID

AB_3096794

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

  • FC

  • 1:1,000

Target

Function

Kinase suppressor of Ras 1 is an enzyme that in humans is encoded by the KSR1 gene. Enables 14-3-3 protein binding activity; ATP binding activity; and protein C-terminus binding activity. Involved in positive regulation of MAPK cascade. Located in endoplasmic reticulum and membrane. Part of protein-containing complex. Implicated in breast adenocarcinoma. Biomarker of breast cancer.

Background References

1. Rao C et al. KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition. Elife. 2021 May

2. Zheng L et al. ELK4 promotes the development of gastric cancer by inducing M2 polarization of macrophages through regulation of the KDM5A-PJA2-KSR1 axis. J Transl Med. 2021 Aug

Subcellular Location

Cytoplasm, Membrane, Cell membrane, Cell projection, ruffle membrane, Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q8IVT5 Human

SwissProt: Q61097 Mouse

Entrez Gene: 360573 Rat

Synonyms

BKSR 1 antibody

BKSR1 antibody

dKsr antibody

EK 31 antibody

EK31 antibody

hb antibody

Kinase suppressor of Ras 1 antibody

Kinase suppressor of ras antibody

KSR 1 antibody

KSR antibody

Expand

Images

  • Western blot analysis of KSR1 on different lysates with Rabbit anti-KSR1 antibody (<a href="/products/HA721963" style="font-weight: bold;text-decoration: underline;">HA721963</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HDLM-2 cell lysate<br />Lane 3: 293T cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: COS-1 cell lysate<br />Lane 6: C2C12 cell lysate<br />Lane 7: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 102 kDa<br />Observed band size: 120/100 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721963" style="font-weight: bold;text-decoration: underline;">HA721963</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KSR1 on different lysates with Rabbit anti-KSR1 antibody (HA721963) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HDLM-2 cell lysate
    Lane 3: 293T cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: COS-1 cell lysate
    Lane 6: C2C12 cell lysate
    Lane 7: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 102 kDa
    Observed band size: 120/100 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721963) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-KSR1 antibody (<a href="/products/HA721963" style="font-weight: bold;text-decoration: underline;">HA721963</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721963" style="font-weight: bold;text-decoration: underline;">HA721963</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-KSR1 antibody (HA721963) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721963) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of C6 cells labeling KSR1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721963" style="font-weight: bold;text-decoration: underline;">HA721963</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of C6 cells labeling KSR1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721963, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"