PCTAIRE1 Recombinant Rabbit Monoclonal Antibody [JE64-55]
Catalog# HA721964
PCTAIRE1 Recombinant Rabbit Monoclonal Antibody [JE64-55]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PCTAIRE1 Recombinant Rabbit Monoclonal Antibody [JE64-55]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human PCTAIRE1 aa 1-150 / 496.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 56 kDa
Positive Control
HepG2 cell lysate, Neuro-2a cell lysate, SH-SY5Y cell lysate, U-87 MG cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat pancreas tissue lysate.
Conjugation
unconjugated
Clone Number
JE64-55
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Serine/threonine-protein kinase PCTAIRE-1 is an enzyme that in humans is encoded by the PCTK1 gene. The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells. This gene is thought to escape X inactivation. Two transcript variants encoding the same protein have been found for this gene. PCTK1 has been shown to interact with CDK5R1.
Background References
1. Gillani SQ et al. PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signals. J Cell Sci. 2022 Feb
2. Gillani SQ et al. Regulation of PCTAIRE1 protein stability by AKT1, LKB1 and BRCA1. Cell Signal. 2021 Sep
Subcellular Location
Cytoplasm, Cytoplasmic vesicle, secretory vesicle, Cell membrane, Synapse, synaptosome.
Synonyms
Cdk16 antibody
CDK16_HUMAN antibody
Cell division protein kinase 16 antibody
Cyclin-dependent kinase 16 antibody
PCTAIRE antibody
PCTAIRE protein kinase 1 antibody
PCTAIRE-motif protein kinase 1 antibody
PCTAIRE1 antibody
PCTGAIRE antibody
PCTK1 antibody
ExpandImages
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Western blot analysis of PCTAIRE1 on different lysates with Rabbit anti-PCTAIRE1 antibody (HA721964) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat pancreas tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56/63 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721964) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"