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AER61 Recombinant Rabbit Monoclonal Antibody [JE64-87]

Usd: 385

Specification

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Catalog# HA721973

AER61 Recombinant Rabbit Monoclonal Antibody [JE64-87]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

AER61 Recombinant Rabbit Monoclonal Antibody [JE64-87]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human AER61 aa 1-200 / 527.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 62 kDa

Positive Control

Neuro-2a cell lysate, U-87 MG cell lysate, HeLa cell lysate, Caco-2 cell lysate, HDLM-2 cell lysate, HepG2 cell lysate, mouse brain tissue lysate, RAW264.7 cell lysate, mouse heart tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

JE64-87

RRID

AB_3096524

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

This gene encodes an enzyme that acts in the lumen of the endoplasmic reticulum to catalyze the transfer of N-acetylglucosamine to serine or threonine residues of extracellular-targeted proteins. This enzyme modifies proteins containing eukaryotic growth factor (EGF)-like domains, including the Notch receptor, thereby regulating developmental signalling. Mutations in this gene have been observed in individuals with Adams-Oliver syndrome 4. Alternative splicing results in multiple transcript variants.

Background References

1. Shu Y et al. EOGT Correlated With Immune Infiltration: A Candidate Prognostic Biomarker for Hepatocellular Carcinoma. Front Immunol. 2022 Jan

2. Nauman M et al. EOGT enables residual Notch signaling in mouse intestinal cells lacking POFUT1. Sci Rep. 2023 Oct

Subcellular Location

Endoplasmic reticulum lumen.

UNIPROT

SwissProt: Q5NDL2 Human

SwissProt: Q8BYW9 Mouse

SwissProt: Q5NDL0 Rat

Synonyms

AER61 glycosyltransferase antibody

AOS4 antibody

C3orf64 antibody

EGF domain specific O linked N acetylglucosamine transferase antibody

EGF domain-specific O-linked N-acetylglucosamine transferase antibody

EGF O GlcNAc transferase antibody

EGF-O-GlcNAc transferase antibody

EOGT antibody

EOGT_HUMAN antibody

EOGT1 antibody

Expand

Images

  • Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (<a href="/products/HA721973" style="font-weight: bold;text-decoration: underline;">HA721973</a>) at 1/2,000 dilution.<br /><br />Lane 1: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 2: U-87 MG cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (20 µg/Lane)<br />Lane 4: Caco-2 cell lysate (20 µg/Lane)<br />Lane 5: HDLM-2 cell lysate (20 µg/Lane)<br />Lane 6: HepG2 cell lysate (20 µg/Lane)<br />Lane 7: Mouse brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 62 kDa<br />Observed band size: 62 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721973" style="font-weight: bold;text-decoration: underline;">HA721973</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (HA721973) at 1/2,000 dilution.

    Lane 1: Neuro-2a cell lysate (20 µg/Lane)
    Lane 2: U-87 MG cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (20 µg/Lane)
    Lane 4: Caco-2 cell lysate (20 µg/Lane)
    Lane 5: HDLM-2 cell lysate (20 µg/Lane)
    Lane 6: HepG2 cell lysate (20 µg/Lane)
    Lane 7: Mouse brain tissue lysate (40 µg/Lane)

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (<a href="/products/HA721973" style="font-weight: bold;text-decoration: underline;">HA721973</a>) at 1/2,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 2: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 3: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 4: Rat kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 62 kDa<br />Observed band size: 62 kDa<br /><br />Exposure time: 1 minute 59 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721973" style="font-weight: bold;text-decoration: underline;">HA721973</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (HA721973) at 1/2,000 dilution.

    Lane 1: RAW264.7 cell lysate (20 µg/Lane)
    Lane 2: Mouse heart tissue lysate (40 µg/Lane)
    Lane 3: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 4: Rat kidney tissue lysate (40 µg/Lane)

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

    Exposure time: 1 minute 59 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation