AER61 Recombinant Rabbit Monoclonal Antibody [JE64-87] (HA721973)
Catalog# HA721973
Rabbit Monoclonal to AER61
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (HA721973) at 1/2,000 dilution.
Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Lane 5: HDLM-2 cell lysate (20 µg/Lane)
Lane 6: HepG2 cell lysate (20 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Lane 5: HDLM-2 cell lysate (20 µg/Lane)
Lane 6: HepG2 cell lysate (20 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.