MAPK6 / ERK3 Recombinant Rabbit Monoclonal Antibody [PSH03-39]
Catalog# HA721984
MAPK6 / ERK3 Recombinant Rabbit Monoclonal Antibody [PSH03-39]
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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HA750873
不含抗保成分
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unconjugated
Overview
Product Name
MAPK6 / ERK3 Recombinant Rabbit Monoclonal Antibody [PSH03-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MAPK6 aa 1-350 / 721.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 83 kDa
Positive Control
A431 cell lysate, A549 cell lysate, PC-3M cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, 293T, C2C12, C6.
Conjugation
unconjugated
Clone Number
PSH03-39
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
Mitogen-activated protein kinase 6 is an enzyme that in humans is encoded by the MAPK6 gene. The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases, also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Background References
1. Jin Y et al. Inactivation of EGLN3 hydroxylase facilitates Erk3 degradation via autophagy and impedes lung cancer growth. Oncogene. 2022 Mar
2. Bogucka K et al. ERK3/MAPK6 is required for KRAS-mediated NSCLC tumorigenesis. Cancer Gene Ther. 2021 May
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
ERK-3 antibody
ERK3 antibody
Extracellular signal regulated kinase 3 antibody
Extracellular signal regulated kinase p97 antibody
Extracellular signal-regulated kinase 3 antibody
HsT17250 antibody
MAP kinase 6 antibody
MAP kinase isoform p97 antibody
MAPK 6 antibody
MAPK6 antibody
ExpandImages
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Western blot analysis of MAPK6 / ERK3 on different lysates with Rabbit anti-MAPK6 / ERK3 antibody (HA721984) at 1/2,000 dilution.
Lane 1: A431 cell lysate
Lane 2: A549 cell lysate
Lane 3: PC-3M cell lysate
Lane 4: C2C12 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 5 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721984, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 83 kDa
Observed band size: 105 kDa -
☑ Knockdown (KD)
Western blot analysis of MAPK6 / ERK3 on different lysates with Rabbit anti-MAPK6 / ERK3 antibody (HA721984) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-MAPK6 / ERK3 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 100 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721984) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of 293T cells labeling MAPK6 / ERK3 with Rabbit anti-MAPK6 / ERK3 antibody (HA721984) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MAPK6 / ERK3 antibody (HA721984) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C2C12 cells labeling MAPK6 / ERK3 with Rabbit anti-MAPK6 / ERK3 antibody (HA721984) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MAPK6 / ERK3 antibody (HA721984) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling MAPK6 / ERK3 with Rabbit anti-MAPK6 / ERK3 antibody (HA721984) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MAPK6 / ERK3 antibody (HA721984) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of 293T cells labeling MAPK6 / ERK3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721984, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of C6 cells labeling MAPK6 / ERK3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721984, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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