MAPK6/ERK3 Recombinant Rabbit Monoclonal Antibody [PSH03-39] (HA721984)
Catalog# HA721984
Rabbit Monoclonal to MAPK6/ERK3
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MAPK6/ERK3 on different lysates with Rabbit anti-MAPK6/ERK3 antibody (HA721984) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: PC-3M cell lysate
Lane 5: C2C12 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 105 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721984) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: PC-3M cell lysate
Lane 5: C2C12 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 105 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721984) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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