Dnmt2 Recombinant Rabbit Monoclonal Antibody [PSH03-43]
Catalog# HA721988
Dnmt2 Recombinant Rabbit Monoclonal Antibody [PSH03-43]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Green monkey
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HA750877
不含抗保成分
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unconjugated
Overview
Product Name
Dnmt2 Recombinant Rabbit Monoclonal Antibody [PSH03-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Dnmt2 aa 1-391 / 391.
Species Reactivity
Human, Green monkey
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 45 kDa
Positive Control
MDA-MB-231 cell lysate, HeLa cell lysate, 293T cell lysate, HCT 116 cell lysate, U-2 OS cell lysate, COS-1 cell lysate, MCF7, human testis tissue.
Conjugation
unconjugated
Clone Number
PSH03-43
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:100
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IHC-P
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1:2,000
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FC
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1:1,000
Target
Function
tRNA (cytosine-5-)-methyltransferase is an enzyme that in humans is encoded by the TRDMT1 gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a protein with similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome. Alternative splicing results in multiple transcript variants encoding different isoforms. It has been shown that human DNMT2 does not methylate DNA but instead methylates cytosine 38 in the anticodon loop of aspartic acid transfer RNA (tRNA(Asp)).
Background References
1. Adamczyk-Grochala J et al. DNMT2/TRDMT1 gene knockout compromises doxorubicin-induced unfolded protein response and sensitizes cancer cells to ER stress-induced apoptosis. Apoptosis. 2023 Feb
2. Li H et al. Restricted tRNA methylation by intermolecular disulfide bonds in DNMT2/TRDMT1. Int J Biol Macromol. 2023 Aug
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
dDNMT antibody
DmMT 2 antibody
DmMT2 antibody
DNA (cytosine 5 ) methyltransferase 2 antibody
DNA (cytosine 5) methyltransferase like protein 2 antibody
DNA (cytosine-5)-methyltransferase-like protein 2 antibody
DNA 5 cytosine methyltransferase antibody
DNA methyltransferase 2 antibody
DNA methyltransferase homolog HsaIIP antibody
DNA MTase homolog HsaIIP antibody
ExpandImages
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Western blot analysis of Dnmt2 on different lysates with Rabbit anti-Dnmt2 antibody (HA721988) at 1/2,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: HeLa cell lysate
Lane 3: 293T cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: U-2 OS cell lysate
Lane 6: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 42 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721988) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Dnmt2 on different lysates with Rabbit anti-Dnmt2 antibody (HA721988) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Dnmt2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 42 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721988) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of MCF7 cells labeling Dnmt2 with Rabbit anti-Dnmt2 antibody (HA721988) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dnmt2 antibody (HA721988) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Dnmt2 antibody (HA721988) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721988) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of MCF7 cells labeling Dnmt2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721988, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"