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Glutathione Reductase Recombinant Rabbit Monoclonal Antibody [JE31-42]

Usd: 385

Specification

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Catalog# HA721989

Glutathione Reductase Recombinant Rabbit Monoclonal Antibody [JE31-42]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Glutathione Reductase Recombinant Rabbit Monoclonal Antibody [JE31-42]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Glutathione Reductase aa 473-522 / 522.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 56 kDa

Positive Control

HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, Jurkat cell lysate, mouse liver tissue lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat lung tissue lysate, rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

JE31-42

RRID

AB_3096778

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Glutathione plays a key role in maintaining proper function and preventing oxidative stress in human cells. It can act as a scavenger for hydroxyl radicals, singlet oxygen, and various electrophiles. Reduced glutathione reduces the oxidized form of the enzyme glutathione peroxidase, which in turn reduces hydrogen peroxide (H2O2), a dangerously reactive species within the cell. In addition, it plays a key role in the metabolism and clearance of xenobiotics, acts as a cofactor in certain detoxifying enzymes, participates in transport, and regenerates antioxidants such and Vitamins E and C to their reactive forms. The ratio of GSSG/GSH present in the cell is a key factor in properly maintaining the oxidative balance of the cell, that is, it is critical that the cell maintains high levels of the reduced glutathione and a low level of the oxidized glutathione disulfide. This narrow balance is maintained by glutathione reductase, which catalyzes the reduction of GSSG to GSH.

Background References

1. Xu FL et al. SLC27A5 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by downregulating glutathione reductase. Cell Death Dis. 2023 Jan

2. Ehtiati S et al. Glutathione reductase system changes in HTLV-1 infected patients. Virusdisease. 2022 Mar

Subcellular Location

Mitochondrion; Cytoplasm.

UNIPROT

SwissProt: P00390 Human

SwissProt: P47791 Mouse

SwissProt: P70619 Rat

Synonyms

GLR1 antibody

GLUR antibody

Glutathione reductase antibody

Glutathione reductase mitochondrial antibody

Glutathione reductase, mitochondrial antibody

GR antibody

Gr1 antibody

GRase antibody

GRD 1 antibody

GRD1 antibody

Expand

Images

  • Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (<a href="/products/HA721989" style="font-weight: bold;text-decoration: underline;">HA721989</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: K-562 cell lysate (20 µg/Lane)<br />Lane 4: Jurkat cell lysate (20 µg/Lane)<br />Lane 5: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 7: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 8: Rat lung tissue lysate (40 µg/Lane)<br />Lane 9: Rat kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 56 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721989" style="font-weight: bold;text-decoration: underline;">HA721989</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (HA721989) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: K-562 cell lysate (20 µg/Lane)
    Lane 4: Jurkat cell lysate (20 µg/Lane)
    Lane 5: Mouse liver tissue lysate (40 µg/Lane)
    Lane 6: Mouse brain tissue lysate (40 µg/Lane)
    Lane 7: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 8: Rat lung tissue lysate (40 µg/Lane)
    Lane 9: Rat kidney tissue lysate (40 µg/Lane)

    Predicted band size: 56 kDa
    Observed band size: 54 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (<a href="/products/HA721989" style="font-weight: bold;text-decoration: underline;">HA721989</a>) at 1/2,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si Glutathione Reductase cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721989" style="font-weight: bold;text-decoration: underline;">HA721989</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (HA721989) at 1/2,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si Glutathione Reductase cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 54 kDa

    Exposure time: 20 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"