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DOCK2 Recombinant Rabbit Monoclonal Antibody [JE53-62]

Usd: 385

Specification

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Catalog# HA721993

DOCK2 Recombinant Rabbit Monoclonal Antibody [JE53-62]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

DOCK2 Recombinant Rabbit Monoclonal Antibody [JE53-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human DOCK2 aa 1,781-1,830 / 1,830.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 212 kDa

Positive Control

Jurkat cell lysate, THP-1 cell lysate, Raji cell lysate.

Conjugation

unconjugated

Clone Number

JE53-62

RRID

AB_3096775

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Dedicator of cytokinesis protein 2 (Dock2) is a protein encoded in the human by the DOCK2 gene. Dock2 is a large (~180 kDa) protein involved in intracellular signalling networks. It is a member of the DOCK-A subfamily of the DOCK family of guanine nucleotide exchange factors (GEFs) which function as activators of small G-proteins. Dock2 specifically activates isoforms of the small G protein Rac.

Background References

1. Namkoong H et al. DOCK2 is involved in the host genetics and biology of severe COVID-19. Nature. 2022 Sep

2. Wang S et al. SULT2B1-CS-DOCK2 axis regulates effector T-cell exhaustion in HCC microenvironment. Hepatology. 2023 Oct

Subcellular Location

Endomembrane system, Cytoplasm, cytoskeleton.

UNIPROT

SwissProt: Q92608 Human

Synonyms

Dedicator of cyto kinesis 2 antibody

Dedicator of cytokinesis 2 antibody

Dedicator of cytokinesis protein 2 antibody

DOCK 2 antibody

FLJ46592 antibody

IMD40 antibody

KIAA0209 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of DOCK2 on different lysates with Rabbit anti-DOCK2 antibody (<a href="/products/HA721993" style="font-weight: bold;text-decoration: underline;">HA721993</a>) at 1/2,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: THP-1 cell lysate<br />Lane 3: Raji cell lysate<br />Lane 4: 293T cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 212 kDa<br />Observed band size: 180 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721993" style="font-weight: bold;text-decoration: underline;">HA721993</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of DOCK2 on different lysates with Rabbit anti-DOCK2 antibody (HA721993) at 1/2,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: Raji cell lysate
    Lane 4: 293T cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 212 kDa
    Observed band size: 180 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721993) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"