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Substance P Recombinant Rabbit Monoclonal Antibody [JE59-73]

Usd: 385

Specification

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Catalog# HA721995

Substance P Recombinant Rabbit Monoclonal Antibody [JE59-73]

Application

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Substance P Recombinant Rabbit Monoclonal Antibody [JE59-73]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Substance P aa 41-90 / 129.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 15 kDa

Positive Control

Rat spinal cord tissue.

Conjugation

unconjugated

Clone Number

JE59-73

RRID

AB_3096817

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200

Target

Function

Preprotachykinin-1, (abbreviated PPT-1, PPT-I, or PPT-A), is a precursor protein that in humans is encoded by the TAC1 gene. The protein has four isoforms—alpha-, beta-, gamma-, and delta-PPT—which can variably undergo post-translational modification to produce neurokinin A (formerly known as substance K) and substance P. Alpha- and delta-PPT can only be modified to substance P, whereas beta- and gamma-PPT can produce both substance P and neurokinin A. Neurokinin A can also be further modified to produce neuropeptide K (also known as neurokinin K) and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Alternative splicing of exons 4 and/or 6 produces four known products of undetermined significance.

Background References

1. Perner C et al. Substance P Release by Sensory Neurons Triggers Dendritic Cell Migration and Initiates the Type-2 Immune Response to Allergens. Immunity. 2020 Nov

2. Ko KR et al. Substance P, A Promising Therapeutic Target in Musculoskeletal Disorders. Int J Mol Sci. 2022 Feb

Subcellular Location

Secreted.

UNIPROT

SwissProt: P20366 Human

SwissProt: P41539 Mouse

SwissProt: P06767 Rat

Synonyms

C-terminal-flanking peptide antibody

Hs.2563 antibody

neurokinin 1 antibody

neurokinin 2 antibody

neurokinin A antibody

neurokinin alpha antibody

Neuromedin L antibody

neuropeptide gamma antibody

neuropeptide K antibody

NK2 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rabbit anti-Substance P antibody (<a href="/products/HA721995" style="font-weight: bold;text-decoration: underline;">HA721995</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721995" style="font-weight: bold;text-decoration: underline;">HA721995</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rabbit anti-Substance P antibody (HA721995) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721995) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation