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PRMT7 Recombinant Rabbit Monoclonal Antibody [JE64-78]

Usd: 385

Specification

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Catalog# HA721996

PRMT7 Recombinant Rabbit Monoclonal Antibody [JE64-78]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PRMT7 Recombinant Rabbit Monoclonal Antibody [JE64-78]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PRMT7 aa 1-50 / 692.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 78 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, HEK-293 cell lysate, 293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

JE64-78

RRID

AB_3096806

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Protein arginine methyltransferase 7 is a protein that in humans is encoded by the PRMT7 gene. Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing.

Background References

1. Liu C et al. Loss of PRMT7 reprograms glycine metabolism to selectively eradicate leukemia stem cells in CML. Cell Metab. 2022 Jun 7

2. Srour N et al. PRMT7 ablation stimulates anti-tumor immunity and sensitizes melanoma to immune checkpoint blockade. Cell Rep. 2022 Mar

Subcellular Location

Cytoplasm, cytosol, Nucleus

UNIPROT

SwissProt: Q9NVM4 Human

SwissProt: Q922X9 Mouse

SwissProt: Q5U4E8 Rat

Synonyms

[Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody

ANM7_HUMAN antibody

FLJ10640 antibody

Histone-arginine N-methyltransferase PRMT7 antibody

KIAA1933 antibody

Myelin basic protein arginine N methyltransferase antibody

OTTHUMP00000174863 antibody

PRMT7 antibody

Protein arginine methyltransferase 7 antibody

Protein arginine N-methyltransferase 7 antibody

Images

  • Western blot analysis of PRMT7 on different lysates with Rabbit anti-PRMT7 antibody (<a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (15 µg/Lane)<br />Lane 2: Jurkat cell lysate (15 µg/Lane)<br />Lane 3: HepG2 cell lysate (15 µg/Lane)<br />Lane 4: HEK-293 cell lysate (15 µg/Lane)<br />Lane 5: 293T cell lysate (15 µg/Lane)<br />Lane 6: MCF7 cell lysate (15 µg/Lane)<br />Lane 7: NIH/3T3 cell lysate (15 µg/Lane)<br />Lane 8: Mouse testis tissue lysate (30 µg/Lane)<br />Lane 9: Rat testis tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 78 kDa<br />Observed band size: 78 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PRMT7 on different lysates with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (15 µg/Lane)
    Lane 2: Jurkat cell lysate (15 µg/Lane)
    Lane 3: HepG2 cell lysate (15 µg/Lane)
    Lane 4: HEK-293 cell lysate (15 µg/Lane)
    Lane 5: 293T cell lysate (15 µg/Lane)
    Lane 6: MCF7 cell lysate (15 µg/Lane)
    Lane 7: NIH/3T3 cell lysate (15 µg/Lane)
    Lane 8: Mouse testis tissue lysate (30 µg/Lane)
    Lane 9: Rat testis tissue lysate (30 µg/Lane)

    Predicted band size: 78 kDa
    Observed band size: 78 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721996) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PRMT7 antibody (<a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PRMT7 antibody (<a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PRMT7 was immunoprecipitated from 0.2 mg mouse testis tissue lysate with <a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: mouse testis tissue lysate (input)<br />Lane 2: <a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a> IP in mouse testis tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA721996" style="font-weight: bold;text-decoration: underline;">HA721996</a> in mouse testis tissue lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 11 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    PRMT7 was immunoprecipitated from 0.2 mg mouse testis tissue lysate with HA721996 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721996 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: mouse testis tissue lysate (input)
    Lane 2: HA721996 IP in mouse testis tissue lysate
    Lane 3: Rabbit IgG instead of HA721996 in mouse testis tissue lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 11 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation