TIRAP Recombinant Rabbit Monoclonal Antibody [JE46-35]
Catalog# HA722001
TIRAP Recombinant Rabbit Monoclonal Antibody [JE46-35]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
TIRAP Recombinant Rabbit Monoclonal Antibody [JE46-35]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human TIRAP aa 1-50 / 221.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 24 kDa
Positive Control
HeLa cell lysate, U-2 OS cell lysate, RAW264.7 cell lysate, mouse liver tissue lysate.
Conjugation
unconjugated
Clone Number
JE46-35
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
TIRAP (TIR domain containing adaptor protein) is an adapter molecule associated with toll-like receptors. The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.
Background References
1. Rajpoot S et al. TIRAP in the Mechanism of Inflammation. Front Immunol. 2021 Jul
2. Lannoy V et al. TIRAP, TRAM, and Toll-Like Receptors: The Untold Story. Mediators Inflamm. 2023 Mar
Subcellular Location
Cytoplasm, Cell membrane, Membrane.
Synonyms
Adapter protein wyatt antibody
FLJ42305 antibody
MAL antibody
MyD88 adapter like protein antibody
TIR domain containing adapter protein antibody
Toll interleukin 1 receptor (TIR) domain containing adaptor protein antibody
Toll interleukin 1 receptor domain containing adapter protein antibody
Toll like receptor adaptor protein antibody
Wyatt antibody
Images
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Western blot analysis of TIRAP on different lysates with Rabbit anti-TIRAP antibody (HA722001) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: U-2 OS cell lysate (20 µg/Lane)
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722001) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"