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CBX2 Recombinant Rabbit Monoclonal Antibody [JE34-45]

Usd: 385

Specification

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Catalog# HA722027

CBX2 Recombinant Rabbit Monoclonal Antibody [JE34-45]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CBX2 Recombinant Rabbit Monoclonal Antibody [JE34-45]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human CBX2 aa 433-532 / 532.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 56 kDa

Positive Control

HEK-293 cell lysate, HeLa cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse heart tissue lysate, HeLa.

Conjugation

unconjugated

Clone Number

JE34-45

RRID

AB_3096837

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Background References

1. Wang B et al. CBX2 Expression in Colorectal Mucosa-adenoma-adenocarcinoma Sequence. J Coll Physicians Surg Pak. 2021 Sep

2. Del Gaudio N et al. CBX2 shapes chromatin accessibility promoting AML via p38 MAPK signaling pathway. Mol Cancer. 2022 Jun

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: Q14781 Human

SwissProt: P30658 Mouse

Entrez Gene: 303730 Rat

Synonyms

Cbx 2 antibody

CBX2 antibody

CBX2_HUMAN antibody

CDC A6 antibody

CDCA6 antibody

Cell division cycle associated 6 antibody

Cell division cycle associated6 antibody

chromobox homolog 2 (Pc class homolog, Drosophila) antibody

Chromobox homolog 2 antibody

Chromobox homolog2 antibody

Expand

Images

  • Western blot analysis of CBX2 on different lysates with Rabbit anti-CBX2 antibody (<a href="/products/HA722027" style="font-weight: bold;text-decoration: underline;">HA722027</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: C2C12 cell lysate<br />Lane 4: Neuro-2a cell lysate<br />Lane 5: NIH/3T3 cell lysate<br />Lane 6: C6 cell lysate<br />Lane 7: Mouse heart tissue lysate<br /><br />Lysates/proteins at 25 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 2 minutes 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722027" style="font-weight: bold;text-decoration: underline;">HA722027</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CBX2 on different lysates with Rabbit anti-CBX2 antibody (HA722027) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: C2C12 cell lysate
    Lane 4: Neuro-2a cell lysate
    Lane 5: NIH/3T3 cell lysate
    Lane 6: C6 cell lysate
    Lane 7: Mouse heart tissue lysate

    Lysates/proteins at 25 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 60 kDa

    Exposure time: 2 minutes 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722027) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HeLa cells labeling CBX2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722027" style="font-weight: bold;text-decoration: underline;">HA722027</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling CBX2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722027, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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