pro Caspase-10 Recombinant Rabbit Monoclonal Antibody [JE59-42]
Catalog# HA722029
pro Caspase-10 Recombinant Rabbit Monoclonal Antibody [JE59-42]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
pro Caspase-10 Recombinant Rabbit Monoclonal Antibody [JE59-42]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Caspase-10 aa 181-280 / 521.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 59 kDa
Positive Control
HepG2 cell lysate, A431 cell lysate, K-562 cell lysate, human breast cancer tissue.
Conjugation
unconjugated
Clone Number
JE59-42
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:2,000
Target
Function
Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. That protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Background References
1. Cho M et al. Caspase-10 affects the pathogenesis of primary biliary cholangitis by regulating inflammatory cell death. J Autoimmun. 2022 Dec
2. Lamarque M et al. Role of Caspase-10-P13tBID axis in erythropoiesis regulation. Cell Death Differ. 2023 Jan
Subcellular Location
Cytoplasm, cytosol, ripoptosome.
UNIPROT
Synonyms
Apoptotic protease Mch4 antibody
CASP10 antibody
Caspase 10 antibody
FLICE2 antibody
ICE like apoptotic protease 4 antibody
Images
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Western blot analysis of pro Caspase-10 on different lysates with Rabbit anti-pro Caspase-10 antibody (HA722029) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: A431 cell lysate
Lane 3: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722029) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-pro Caspase-10 antibody (HA722029) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722029) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"