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MYCBP2 Recombinant Rabbit Monoclonal Antibody [PSH03-68]

Usd: 385

Specification

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Catalog# HA722030

MYCBP2 Recombinant Rabbit Monoclonal Antibody [PSH03-68]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

MYCBP2 Recombinant Rabbit Monoclonal Antibody [PSH03-68]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MYCBP2 aa 1-300.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 514 kDa

Positive Control

HeLa cell lysate, THP-1 cell lysate, HUVEC cell lysate, HEK-293 cell lysate, A431 cell lysate, mouse brain tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, HeLa, human brain tissue.

Conjugation

unconjugated

Clone Number

PSH03-68

RRID

AB_3096644

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:500

  • FC

  • 1:1,000

Target

Function

Probable E3 ubiquitin-protein ligase MYCBP2 also known as myc-binding protein 2 or protein associates with myc (PAM) is an enzyme that in humans is encoded by the MYCBP2 gene. PAM contains a N-terminal leucine zipper, central MYC-binding, and C-terminal histone-binding protein homology domains. MYCBP2 has been shown to interact with Myc.

Background References

1. AlAbdi L et al. Loss-of-function variants in MYCBP2 cause neurobehavioural phenotypes and corpus callosum defects. Brain. 2023 Apr

2. Wang G et al. MYCBP2 expression correlated with inflammatory cell infiltration and prognosis immunotherapy in thyroid cancer patients. Front Immunol. 2022 Dec

Subcellular Location

Nucleus, Cell projection, axon, Cytoplasm, cytoskeleton.

UNIPROT

SwissProt: O75592 Human

SwissProt: Q7TPH6 Mouse

Entrez Gene: 290447 Rat

Synonyms

AU023734 antibody

AW107953 antibody

AW546647 antibody

C130061D10Rik antibody

DKFZp686M08244 antibody

FLJ10106 antibody

FLJ13826 antibody

FLJ21597 antibody

FLJ21646 antibody

KIAA0916 antibody

Expand

Images

  • Western blot analysis of MYCBP2 on different lysates with Rabbit anti-MYCBP2 antibody (<a href="/products/HA722030" style="font-weight: bold;text-decoration: underline;">HA722030</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: THP-1 cell lysate (20 µg/Lane)<br />Lane 3: HUVEC cell lysate (20 µg/Lane)<br />Lane 4: HEK-293 cell lysate (20 µg/Lane)<br />Lane 5: A431 cell lysate (20 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 7: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 8: Rat brain tissue lysate (40 µg/Lane)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 514 kDa<br />Observed band size: 514 kDa<br /><br />Exposure time: 1 minute;<br /><br />3-8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722030" style="font-weight: bold;text-decoration: underline;">HA722030</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MYCBP2 on different lysates with Rabbit anti-MYCBP2 antibody (HA722030) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: THP-1 cell lysate (20 µg/Lane)
    Lane 3: HUVEC cell lysate (20 µg/Lane)
    Lane 4: HEK-293 cell lysate (20 µg/Lane)
    Lane 5: A431 cell lysate (20 µg/Lane)
    Lane 6: Mouse brain tissue lysate (40 µg/Lane)
    Lane 7: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 8: Rat brain tissue lysate (40 µg/Lane)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 514 kDa
    Observed band size: 514 kDa

    Exposure time: 1 minute;

    3-8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722030) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling MYCBP2 with Rabbit anti-MYCBP2 antibody (<a href="/products/HA722030" style="font-weight: bold;text-decoration: underline;">HA722030</a>) at 1/100 dilution.<br /><br />Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MYCBP2 antibody (<a href="/products/HA722030" style="font-weight: bold;text-decoration: underline;">HA722030</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling MYCBP2 with Rabbit anti-MYCBP2 antibody (HA722030) at 1/100 dilution.

    Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MYCBP2 antibody (HA722030) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MYCBP2 antibody (<a href="/products/HA722030" style="font-weight: bold;text-decoration: underline;">HA722030</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722030" style="font-weight: bold;text-decoration: underline;">HA722030</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MYCBP2 antibody (HA722030) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722030) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of HeLa cells labeling MYCBP2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722030" style="font-weight: bold;text-decoration: underline;">HA722030</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling MYCBP2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722030, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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