HMGB3 Recombinant Rabbit Monoclonal Antibody [JE63-39]
Catalog# HA722033
HMGB3 Recombinant Rabbit Monoclonal Antibody [JE63-39]
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WB
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Human
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unconjugated
Overview
Product Name
HMGB3 Recombinant Rabbit Monoclonal Antibody [JE63-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human HMG4 aa 50-150.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 23 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, Jurkat cell lysate.
Conjugation
unconjugated
Clone Number
JE63-39
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling.
Background References
1. Zhou X et al. Overexpression of HMGB3 and its prognostic value in breast cancer. Front Oncol. 2022 Dec
2. Ma H et al. HMGB3 promotes PARP inhibitor resistance through interacting with PARP1 in ovarian cancer. Cell Death Dis. 2022 Mar
Subcellular Location
Nucleus, Chromosome, Cytoplasm.
UNIPROT
Synonyms
chromosomal protein, Nonhistone, HMG4 antibody
High mobility group (nonhistone chromosomal) protein 4 antibody
High mobility group box 3 antibody
High mobility group protein 2a antibody
High mobility group protein 4 antibody
High mobility group protein B3 antibody
High mobility group protein HMG4 antibody
HMG 4 antibody
HMG-2a antibody
HMG-4 antibody
ExpandImages
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Western blot analysis of HMGB3 on different lysates with Rabbit anti-HMGB3 antibody (HA722033) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722033) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"