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GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH03-87]

Usd: 385

Specification

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Catalog# HA722053

GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH03-87]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH03-87]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human GABA B Receptor 1 aa 142-591 / 961.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 108 kDa

Positive Control

Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse cerebellum tissue lysate, rat cerebellum tissue lysate, mouse cerebellum tissue, human cerebellum tissue, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

PSH03-87

RRID

AB_3096571

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:1,000-1:20,000

Target

Function

Gamma-aminobutyric acid B receptor, 1 (GABAB1), is a G-protein coupled receptor subunit encoded by the GABBR1 gene. GABAB1 is a receptor for Gamma-aminobutyric acid. Upon binding, GABAB1 will produce a slow and prolonged inhibitory effect. GABAB1 is one part of a heterodimer, which is the GABAB receptor, consisting of it and the related GABAB2 protein. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants. GABBR1 has been shown to interact with ATF4 and GABBR2.

Background References

1. Shao L et al. The neurotransmitter receptor Gabbr1 regulates proliferation and function of hematopoietic stem and progenitor cells. Blood. 2021 Feb

2. Cediel ML et al. GABBR1 monoallelic de novo variants linked to neurodevelopmental delay and epilepsy. Am J Hum Genet. 2022 Oct

Subcellular Location

Cell membrane, Postsynaptic cell membrane, Cell projection, dendrite; Secreted.

UNIPROT

SwissProt: Q9UBS5 Human

SwissProt: Q9WV18 Mouse

SwissProt: Q9Z0U4 Rat

Synonyms

dJ271M21.1.1 antibody

dJ271M21.1.2 antibody

FLJ92613 antibody

GABA-B receptor 1 antibody

GABA-B-R1 antibody

GABA-BR1 antibody

GABAB R1 antibody

GABAB subunit 1c antibody

GABABR1 antibody

GABBR1 3 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: Human brain tissue lysate<br />Lane 2: Mouse brain tissue lysate (hot lysis)<br />Lane 3: Rat brain tissue lysate (no heat)<br />Lane 4: Mouse cerebellum tissue lysate (70℃ heat)<br />Lane 5: Rat cerebellum tissue lysate<br />Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative)<br />Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 108 kDa<br />Observed band size: 95-108 kDa<br /><br />Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: Human brain tissue lysate
    Lane 2: Mouse brain tissue lysate (hot lysis)
    Lane 3: Rat brain tissue lysate (no heat)
    Lane 4: Mouse cerebellum tissue lysate (70℃ heat)
    Lane 5: Rat cerebellum tissue lysate
    Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative)
    Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 108 kDa
    Observed band size: 95-108 kDa

    Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722053, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/5,000 dilution.<br /><br />Lane 1: Human brain tissue lysate (40 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane)<br />Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane)<br />Lane 4: Mouse cerebellum tissue lysate (70℃ heat) (20 µg/Lane)<br />Lane 5: Rat cerebellum tissue lysate (20 µg/Lane)<br />Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane)<br />Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 108 kDa<br />Observed band size: 95-108 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/5,000 dilution.

    Lane 1: Human brain tissue lysate (40 µg/Lane)
    Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane)
    Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane)
    Lane 4: Mouse cerebellum tissue lysate (70℃ heat) (20 µg/Lane)
    Lane 5: Rat cerebellum tissue lysate (20 µg/Lane)
    Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane)
    Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 108 kDa
    Observed band size: 95-108 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722053) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722053) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722053" style="font-weight: bold;text-decoration: underline;">HA722053</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722053) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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